Lambda Light Chain Antibody rLLC/1738 / IGL Antibody [clone rLLC/1738] (V7878)

Lambda Light Chain Antibody rLLC/1738 in Human Spleen Tissue IHC. Immunohistochemistry staining of FFPE human spleen tissue with Lambda Light Chain Antibody clone rLLC/1738 demonstrates heterogeneous brown staining within the splenic parenchyma, with strongly positive individual cells distributed among more weakly stained and unstained cells. HIER was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 min followed by cooling before testing. The heterogeneous cellular pattern is consistent with detection of IGL-expressing cells within human spleen.

Lambda Light Chain Antibody rLLC/1738 with Negative Control in Human Spleen IHC. Immunohistochemistry staining of FFPE human spleen tissue with Lambda Light Chain Antibody clone rLLC/1738 shows heterogeneous brown cellular staining, including scattered strongly positive cells. The PBS negative-control inset, in which primary antibody was omitted, shows little to no corresponding brown staining. HIER was performed using pH 9 10 mM Tris with 1 mM EDTA for 20 min followed by cooling. The comparison supports specific IGL Antibody staining under the tested conditions.

Purified Lambda Light Chain Antibody rLLC/1738 Reducing and Non-Reducing SDS-PAGE. SDS-PAGE analysis of purified, BSA-free Lambda Light Chain Antibody clone rLLC/1738 shows a predominant intact antibody species at approximately 150 kDa under non-reducing conditions. Under reducing conditions, prominent heavy and light antibody chains are visible at approximately 50 and 25 kDa, respectively. The gel provides an assessment of the integrity and composition of the purified IGL Antibody preparation.

Lambda Light Chain Antibody rLLC/1738 in Human Serum WB. Western blot testing of human serum with Lambda Light Chain Antibody clone rLLC/1738 detects a strong, discrete band at approximately 25-27 kDa with minimal additional reactivity. The observed molecular weight is consistent with the expected migration of human immunoglobulin lambda light chain. The predominant signal demonstrates recognition by the IGL Antibody in human serum under the tested conditions.