Lambda Light Chain Antibody rLLC/3774R / Immunoglobulin Lambda Locus Antibody [clone LLC/3774R] (V8873)

IHC staining of FFPE human spleen tissue with recombinant Lambda Light Chain antibody (clone LLC/3774R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human spleen tissue with recombinant Lambda Light Chain (clone LLC/3774R). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant Lambda Light Chain antibody (clone LLC/3774R) as confirmation of integrity and purity.

Lambda Light Chain Antibody rLLC/3774R in Human Serum and Spleen WB. Western blot testing of human serum and spleen tissue lysates with Lambda Light Chain Antibody clone LLC/3774R detects a strong, discrete band at approximately 26-28 kDa in both samples, with minimal additional reactivity. The observed molecular weight is consistent with the expected migration of human immunoglobulin lambda light chains. The clean signal in both serum and spleen demonstrates recognition by the Immunoglobulin Lambda Locus Antibody across soluble and tissue-derived human samples.

Lambda Light Chain Antibody rLLC/3774R Species Specificity in Spleen Lysates WB. Western blot testing of human, mouse, rat, hamster, guinea pig, and rabbit spleen tissue lysates with Lambda Light Chain Antibody clone LLC/3774R detects a strong, discrete band at approximately 26-28 kDa in human spleen, with no corresponding signal detected in the other species tested. The observed band is consistent with the expected migration of human immunoglobulin lambda light chain and demonstrates human-specific recognition by the Immunoglobulin Lambda Locus Antibody under the tested conditions.