Lambda Antibody LLC/3778R / Immunoglobulin Lambda Locus Antibody [clone LLC/3778R] (V7879)

Lambda Antibody LLC/3778R in Human Spleen Tissue IHC. Immunohistochemistry staining of FFPE human spleen tissue with Lambda Antibody clone LLC/3778R demonstrates heterogeneous brown cellular staining, with scattered strongly positive cells distributed throughout the splenic parenchyma. HIER was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 min followed by cooling before testing. The cellular distribution is consistent with detection of lambda light chain-expressing cells by the Immunoglobulin Lambda Locus Antibody.

Lambda Antibody LLC/3778R Detects Lambda-Positive Cells in Human Spleen IHC. Immunohistochemistry staining of FFPE human spleen tissue with Lambda Antibody clone LLC/3778R shows heterogeneous brown staining, including numerous strongly positive individual cells among more weakly stained and unstained cells. HIER was performed using pH 9 10 mM Tris with 1 mM EDTA for 20 min followed by cooling before testing. The distinct cellular staining demonstrates recognition by the Immunoglobulin Lambda Locus Antibody in human spleen.

Purified Lambda Antibody LLC/3778R Reducing and Non-Reducing SDS-PAGE. SDS-PAGE analysis of purified, BSA-free Lambda Antibody clone LLC/3778R shows a predominant intact antibody species at approximately 150 kDa under non-reducing conditions. Under reducing conditions, prominent heavy and light antibody chains are visible at approximately 50 and 25 kDa, respectively. The gel provides an assessment of the integrity and composition of the purified Immunoglobulin Lambda Locus Antibody preparation.

Recombinant Lambda Antibody LLC/3778R in Human Serum and Spleen WB. Western blot testing of human serum and spleen tissue lysates with Recombinant Lambda Antibody clone LLC/3778R detects a strong, discrete band at approximately 26-28 kDa in both samples, with minimal additional reactivity. The observed molecular weight is consistent with the expected migration of human immunoglobulin lambda light chain. The comparable signal in serum and spleen demonstrates recognition by this recombinant rabbit monoclonal antibody across soluble and tissue-derived human samples.

Recombinant Lambda Antibody LLC/3778R Species Specificity in Spleen Lysates WB. Western blot testing of human, mouse, rat, hamster, guinea pig, and rabbit spleen tissue lysates with Recombinant Lambda Antibody clone LLC/3778R detects a strong, discrete band at approximately 26-28 kDa in human spleen, with no corresponding signal detected in the other species tested. The observed band is consistent with the expected migration of human immunoglobulin lambda light chain and demonstrates human-specific recognition by this recombinant rabbit monoclonal antibody under the tested conditions.