KRT8 Antibody / Cytokeratin 8 [clone rKRT8/6471] (V8981)

KRT8 Antibody Human Colon IHC. Immunohistochemical staining of FFPE human colon tissue using recombinant KRT8 Antibody (clone rKRT8/6471) demonstrates strong membranous and cytoplasmic HRP-DAB brown staining within epithelial cells. The staining pattern is consistent with expression of Cytokeratin 8 (KRT8/CK8), a type II intermediate filament protein widely expressed in simple epithelial tissues where it contributes to cytoskeletal organization, epithelial integrity, and cellular stress-response pathways. KRT8 immunoreactivity highlights epithelial cell morphology and tissue architecture within the colon while surrounding stromal elements exhibit comparatively limited staining. HIER: boil tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 minutes and allow to cool before testing.

KRT8 Antibody Human Colon Tissue IHC. Immunohistochemical staining of FFPE human colon tissue using recombinant KRT8 Antibody (clone rKRT8/6471) demonstrates strong cytoplasmic and membranous HRP-DAB brown staining throughout epithelial cell populations. The staining pattern is consistent with expression of Cytokeratin 8 (KRT8/CK8), a type II intermediate filament protein that supports epithelial cytoskeletal organization, structural integrity, and cellular stress-response functions. KRT8 immunoreactivity highlights epithelial morphology and tissue architecture within the colon, while adjacent stromal and lymphoid elements show comparatively limited staining. HIER: boil tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 minutes and allow to cool before testing.

KRT8 Antibody Human Brain Negative Control IHC. Immunohistochemical staining of FFPE human brain tissue using recombinant KRT8 Antibody (clone rKRT8/6471) under negative control conditions demonstrates an absence of specific HRP-DAB staining, with only hematoxylin-counterstained nuclei visible throughout the tissue section. The lack of detectable signal confirms minimal nonspecific background staining and supports the specificity of the immunohistochemical procedure. Cytokeratin 8 (KRT8/CK8) is an epithelial intermediate filament protein that is not expected to be broadly expressed within normal brain parenchyma. Antibody incubation was performed in PBS for 30 minutes at room temperature. HIER: boil tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 minutes and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant KRT8 antibody (clone rKRT8/6471) as confirmation of integrity and purity.