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Home >> Antibodies >> Recombinant KRT7 Antibody / Cytokeratin 7

Recombinant KRT7 Antibody / Cytokeratin 7 [clone rKRT7/8763] (V4281)

  Catalog No Formulation Size Price (USD)  
Image V4281-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V4281-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V4281SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recmousemono
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IHC staining of FFPE human prostate carcinoma tissue with recombinant KRT7 antibody (clone rKRT7/8763). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant KRT7 antibody (clone rKRT7/8763) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG2b, kappa
Clone Name rKRT7/8763
Purity Protein A/G affinity
UniProt P08729
Localization Cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations This recombinant KRT7 antibody is available for research use only.
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Description

It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This mAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract.This mAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).

Application Notes

Optimal dilution of the recombinant KRT7 antibody should be determined by the researcher.

Immunogen

Recombinant full-length human KRT7 protein was used as the immunogen for the recombinant KRT7 antibody.

Storage

Aliquot the recombinant KRT7 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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