KRT10 Antibody rKRT10/844 / Recombinant Keratin 10 Antibody [clone rKRT10/844] (V3603)

KRT10 Antibody rKRT10/844 by WB in Human Skin. Western blot analysis of human skin tissue lysate using KRT10 Antibody rKRT10/844. A prominent immunoreactive band is detected at approximately 56-60 kDa, consistent with the predicted molecular weight of cytokeratin 10 (56.5 kDa). The single sample lane shows a well-defined band with minimal additional immunoreactivity. The Recombinant Keratin 10 Antibody demonstrates detection of endogenous KRT10 in human skin tissue, where this intermediate filament protein is expressed predominantly in differentiated suprabasal keratinocytes.

KRT10 Antibody rKRT10/844 by IHC in Human Skin. Immunohistochemistry of FFPE human skin tissue using KRT10 Antibody rKRT10/844. Strong brown cytoplasmic staining is observed in the suprabasal epidermal layers, with comparatively little staining in the basal keratinocytes and underlying dermis. The Recombinant Keratin 10 Antibody highlights differentiated epidermal cells, consistent with the expected distribution of KRT10 in stratified squamous epithelium. Nuclei are counterstained blue with hematoxylin. Heat-induced epitope retrieval was performed using 10 mM citrate buffer, pH 6, for 10-20 minutes, followed by cooling at room temperature for 20 minutes.

KRT10 Antibody rKRT10/844 by SDS-PAGE. SDS-PAGE analysis of purified KRT10 Antibody rKRT10/844 under non-reducing (NR) and reducing (R) conditions, with 2 ug of antibody loaded per lane. The non-reducing lane shows a prominent band migrating at approximately 130-150 kDa, consistent with intact IgG. Under reducing conditions, distinct bands are observed at approximately 50 kDa and 25 kDa, corresponding to the immunoglobulin heavy and light chains, respectively. The Recombinant Keratin 10 Antibody demonstrates the expected electrophoretic profile of a purified mouse monoclonal antibody preparation.