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Home >> Antibodies >> Recombinant Ki-67 Antibody

Recombinant Ki-67 Antibody [clone rMKI67/6499] (V8961)

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  Catalog No Formulation Size Price (USD)  
Image V8961-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V8961-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V8961SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recmousemono
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IHC staining of FFPE human tonsil tissue with recombinant Ki67 antibody (clone rMKI67/6499). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human tonsil tissue with recombinant Ki67 antibody (clone rMKI67/6499). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Negative control: IHC staining of FFPE human brain tissue using recombinant Ki-67 antibody (clone rMKI67/6499) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant Ki-67 antibody (clone rMKI67/6499) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG2a, kappa
Clone Name rMKI67/6499
Purity Protein A/G affinity
UniProt P46013
Localization Nuclear
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This recombinant Ki-67 antibody is available for research use only.
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Description

Ki-67 antigen is a nuclear, non-histone protein that is present in all stages of the cell cycle except G0. This characteristic makes Ki-67 an excellent marker for proliferating cells and is commonly used as one of the prognostic factors in cancer studies. A correlation has been demonstrated between Ki-67 index and the histo-pathological grade of neoplasms. Assessment of Ki-67 expression in renal and ureter tumors shows a correlation between tumor proliferation and disease progression, thus making it possible to differentiate high-risk patients. Ki-67 expression may also prove to be important for distinguishing between malignant and benign peripheral nerve sheath tumors. Ki-67 labeling index has been shown to be a prognostic marker in a number of neoplasms including grade II astrocytoma, oligodendroglioma, colon carcinoma, and breast carcinoma. In general, Ki-67 is a good marker of proliferating cell populations.

Application Notes

Optimal dilution of the recombinant Ki-67 antibody should be determined by the researcher.

Immunogen

A portion of amino acids 2293-2478 from the human protein was used as the immunogen for the recombinant Ki-67 antibody.

Storage

Aliquot the recombinant Ki-67 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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