Keratin 16 Antibody KRT16/2043R / CK16 Antibody [clone KRT16/2043R] (V3625)

Keratin 16 Antibody KRT16/2043R by WB in human skeletal muscle. Western blot analysis of human skeletal muscle tissue lysate using Keratin 16 Antibody clone KRT16/2043R. The blot contains a single sample lane alongside molecular weight markers. A prominent immunoreactive band is visible near 48-50 kDa, consistent with the predicted molecular weight of human KRT16, approximately 51 kDa. The detected band migrates within the anticipated molecular weight range for this type I intermediate filament protein. The CK16 Antibody recognizes a protein near the expected molecular weight under the tested conditions. However, skeletal muscle is not a characteristic KRT16-expressing tissue, so the identity of the detected band has not been independently established.

Keratin 16 Antibody KRT16/2043R purity by SDS-PAGE. SDS-PAGE analysis of purified, BSA-free Keratin 16 Antibody clone KRT16/2043R under non-reduced (NR) and reduced (R) conditions. The non-reduced sample exhibits a prominent band near 150 kDa, consistent with intact immunoglobulin. Under reduced conditions, distinct bands migrate at approximately 50 kDa and 25 kDa, corresponding to immunoglobulin heavy and light chains. Molecular weight standards are included for reference. This analysis evaluates the electrophoretic characteristics and purity of the CK16 Antibody preparation rather than the detection of endogenous keratin 16.

Keratin 16 Antibody KRT16/2043R by IHC in human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded human skin tissue using Keratin 16 Antibody clone KRT16/2043R. Brown cytoplasmic immunoreactivity is visible throughout a multilayered epithelial region, highlighting the cytoplasm of numerous keratinocytes. The distribution is consistent with the cytoplasmic localization of keratin 16 in stratified epithelial cells. Hematoxylin counterstaining reveals nuclei in blue, providing contrast with the positively stained epithelium. The CK16 Antibody was applied following heat-induced epitope retrieval in 10 mM citrate buffer, pH 6, for 10-20 minutes, followed by cooling at room temperature for 20 minutes.