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Home >> Antibodies >> Recombinant Keratin 10 Antibody

Recombinant Keratin 10 Antibody [clone rKRT10/1275] (V3602)

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  Catalog No Formulation Size Price (USD)  
Image V3602-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3602-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3602SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V3602IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
Microvalidated Recmousemono
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IHC testing of FFPE human skin with recombinant Keratin 10 antibody (clone rKRT10/1275). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
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IHC testing of FFPE human skin with recombinant Keratin 10 antibody (clone rKRT10/1275). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE analysis of purified, BSA-free recombinant Keratin 10 antibody (clone rKRT10/1275) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rKRT10/1275
Purity Protein G affinity chromatography
UniProt P13645
Localization Cytoplasmic
Applications Immunohistochemistry (FFPE) : 0.1-0.2ug/ml for 30 min at RT
Limitations This recombinant Keratin 10 antibody is available for research use only.
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Description

This mAb recognizes a protein of 56.5kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.

Application Notes

Optimal dilution of the recombinant Keratin 10 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human Cytokeratin 10 protein was used as the immunogen for the recombinant Keratin 10 antibody.

Storage

Store the recombinant Keratin 10 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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