IVRN Antibody rIVRN/827 / Involucrin Monoclonal Antibody [clone rIVRN/827] (V7897)

IVRN Antibody rIVRN/827 Western Blot of Human Skin and A431 Cells. Western blot testing of human skin (lane 1) and A431 epidermoid carcinoma cell (lane 2) lysates with IVRN Antibody clone rIVRN/827. A strong predominant band is detected at approximately 130-140 kDa in both samples. Although involucrin has a calculated molecular weight of approximately 68 kDa, its unusual glutamine-rich, repetitive amino acid composition results in characteristically anomalous migration on SDS-PAGE. The comparable target-sized signal in both samples demonstrates endogenous involucrin detection using this Involucrin Monoclonal Antibody.

IVRN Antibody rIVRN/827 Western Blot of Skin from Multiple Species. Western blot testing of human, mouse, rat, hamster, guinea pig, and rabbit skin tissue lysates with IVRN Antibody clone rIVRN/827. A strong predominant band at approximately 130-140 kDa is detected exclusively in human skin, while no corresponding signal is visible in the five non-human samples. The elevated apparent molecular weight is characteristic of involucrin's anomalous SDS-PAGE migration. This result demonstrates strong human specificity and no detectable cross-reactivity with the tested rodent and rabbit skin samples using this Involucrin Monoclonal Antibody.

IVRN Antibody rIVRN/827 in Human Tonsil IHC. Immunohistochemistry testing of FFPE human tonsil tissue with IVRN Antibody clone rIVRN/827. Strong brown cytoplasmic staining is concentrated in the differentiated suprabasal squamous epithelium, while the more basal epithelial region shows substantially weaker staining. The image shows the characteristic differentiation-associated distribution of involucrin within stratified squamous epithelium and demonstrates tissue detection using this Involucrin Monoclonal Antibody.

Purified IVRN Antibody rIVRN/827 SDS-PAGE. SDS-PAGE analysis of purified, BSA-free IVRN Antibody clone rIVRN/827 under non-reducing (NR) and reducing (R) conditions with a 2 ug antibody load. The non-reduced preparation shows a predominant intact antibody band at approximately 150 kDa. Under reducing conditions, the antibody resolves into approximately 50 kDa heavy-chain and 25 kDa light-chain bands. The expected electrophoretic profile demonstrates the integrity of the purified recombinant mouse Involucrin Monoclonal Antibody preparation.