Involucrin Antibody IVRN/2113R / IVL Monoclonal Antibody [clone IVRN/2113R] (V3593)

Involucrin Antibody IVRN/2113R Western Blot of Skin from Multiple Species. Western blot testing of human, mouse, rat, hamster, guinea pig, and rabbit skin tissue lysates with Involucrin Antibody clone IVRN/2113R. A strong predominant band at approximately 130-140 kDa is detected exclusively in human skin, while no corresponding signal is visible in the five non-human samples. Although involucrin has a calculated molecular weight of approximately 68 kDa, it characteristically migrates at a substantially higher apparent molecular weight on SDS-PAGE. The result demonstrates human-specific recognition with no detectable cross-reactivity in the tested non-human skin samples using this IVL Monoclonal Antibody.

Involucrin Antibody IVRN/2113R Western Blot of Human Skin and MCF-7 Cells. Western blot testing of human skin (lane 1) and MCF-7 breast cancer cell (lane 2) lysates with Involucrin Antibody clone IVRN/2113R. A strong predominant band at approximately 130-140 kDa is detected in human skin, while MCF-7 cells show a weaker doublet at a similar apparent molecular weight. The elevated migration relative to involucrin's calculated molecular weight is characteristic of this protein. The target-sized signals demonstrate endogenous involucrin detection in two distinct human sample types using this IVL Monoclonal Antibody.

Involucrin Antibody IVRN/2113R in Human Tonsil IHC. Immunohistochemistry testing of FFPE human tonsil tissue with Involucrin Antibody clone IVRN/2113R. Staining of formalin-fixed tissue is enhanced by digestion with Trypsin or Protease XXV at 1 mg/ml PBS for 5 min at 37oC, which is more effective than citrate buffer-based epitope unmasking for this antibody. Strong brown cytoplasmic staining is concentrated throughout the differentiated suprabasal squamous epithelium, while the adjacent lymphoid tissue shows comparatively little signal. This differentiation-associated epithelial pattern demonstrates detection of involucrin using the IVL Monoclonal Antibody.

Purified Involucrin Antibody IVRN/2113R SDS-PAGE. SDS-PAGE analysis of purified, BSA-free Involucrin Antibody clone IVRN/2113R under non-reducing and reducing conditions. The non-reduced preparation shows the intact antibody at approximately 150 kDa, while reduction produces the expected immunoglobulin heavy and light chains at approximately 50 kDa and 25 kDa. The expected electrophoretic profile provides a quality-control assessment of the purified recombinant rabbit IVL Monoclonal Antibody.