INHA Antibody INHA/6598R / Inhibin Alpha Monoclonal Antibody [clone INHA/6598R] (V9156)

INHA Antibody INHA/6598R Staining in Human Testis by IHC. Immunohistochemistry staining of FFPE human testis using INHA Antibody (clone INHA/6598R) shows strong brown cytoplasmic labeling within seminiferous tubules, while surrounding stromal areas show comparatively little staining. HIER was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min followed by cooling before testing. The distinct intratubular staining demonstrates detection of inhibin alpha in human testis sections using this Inhibin Alpha Monoclonal Antibody.

INHA Antibody INHA/6598R Staining in Human Testicular Carcinoma by IHC. Immunohistochemistry staining of FFPE human testicular carcinoma using INHA Antibody (clone INHA/6598R) at 2 ug/ml shows intense brown cytoplasmic staining in discrete tumor-cell populations, with markedly less staining in the surrounding tissue. HIER was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min followed by cooling before testing. The strong tumor-cell staining demonstrates detection of inhibin alpha using this Inhibin Alpha Monoclonal Antibody.

INHA Antibody INHA/6598R Negative Control in Human Brain by IHC. Immunohistochemistry staining of FFPE human brain using INHA Antibody (clone INHA/6598R) at 2 ug/ml shows essentially no brown staining, providing a negative tissue control for the positive testis and testicular carcinoma results. HIER was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min followed by cooling before testing. The minimal signal observed with this Inhibin Alpha Monoclonal Antibody is consistent with low or absent INHA expression in brain tissue.

INHA Antibody INHA/6598R Purity and Integrity by SDS-PAGE. SDS-PAGE analysis of 2 ug purified, BSA-free INHA Antibody (clone INHA/6598R) shows immunoglobulin heavy and light chains at approximately 50 kDa and 25 kDa under reducing conditions. Under non-reducing conditions, the intact recombinant antibody produces a predominant band above 100 kDa. The expected reducing and non-reducing migration patterns support the integrity and purity of this Inhibin Alpha Monoclonal Antibody preparation.

INHA Antibody INHA/6598R Detection in Human Testis by WB. Western blot analysis of human testis tissue lysate using INHA Antibody (clone INHA/6598R) shows a discrete predominant band at approximately 35-40 kDa, with little visible background. This migration is consistent with the glycosylated INHA precursor, which has a theoretical molecular weight of approximately 40 kDa before proteolytic processing into mature inhibin subunits. The clean predominant band demonstrates detection of inhibin alpha in human testis lysate using this Inhibin Alpha Monoclonal Antibody.