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Home >> Antibodies >> Recombinant IgM Antibody

Recombinant IgM Antibody [clone rIM260] (V7862)

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  Catalog No Formulation Size Price (USD)  
Image V7862-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7862-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7862SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recmousemono
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IHC staining of FFPE human tonsil with recombinant IgM antibody (clone rIM260). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
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IHC staining of FFPE human tonsil with recombinant IgM antibody (clone rIM260). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant IgM antibody (clone rIM260) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rIM260
Purity Protein G affinity chromatography
UniProt P01871
Localization Cytoplasmic, cell surface, secreted
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This recombinant IgM antibody is available for research use only.
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Description

Recognizes a protein of 75kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin s lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

Application Notes

Optimal dilution of the recombinant IgM antibody should be determined by the researcher.

Immunogen

Full length recombinant human IGHM protein was used as the immunogen for the recombinant IgM antibody.

Storage

Store the recombinant IgM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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