- Tel: 858.663.9055
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Recombinant IgM Antibody [clone MuHC2-2R] (V7338)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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V7338-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 100 ug | 519 | |
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V7338-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 20 ug | 229 | |
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V7338SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 519 | |
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V7338IHC-7ML | Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* | 7 ml | 519 |
|
| Availability | 1-3 business days |
| Species Reactivity | Human. Other species not tested. |
| Format | Purified |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG, kappa |
| Clone Name | MuHC2-2R |
| Purity | Protein A affinity chromatography |
| UniProt | P01871 , P20769 |
| Localization | Cytoplasm, cell surface, secreted |
| Applications | Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT Prediluted IHC only format : incubate for 30 min at RT (1) |
| Limitations | This recombinant IgM antibody is available for research use only. |
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The IGHM gene encodes the C region of the mu heavy chain, which defines the IgM isotype. Naive B cells express the transmembrane forms of IgM and IgD on their surface. During an antibody response, activated B cells can switch to the expression of individual downstream heavy chain C region genes by a process of somatic recombination known as isotype switching. In addition, secreted Ig forms that act as antibodies can be produced by alternative RNA processing of the heavy chain C region sequences. Although the membrane forms of all Ig isotypes are monomeric, secreted IgM forms pentamers, and occasionally hexamers, in plasma. [RefSeq]
The optimal dilution of the recombinant IgM antibody for each application should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Recombinant protein was used as the immunogen for this recombinant IgM antibody.
Store the recombinant IgM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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