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Home >> Antibodies >> Recombinant IgM Antibody IGHM/3776R / Recombinant Rabbit Monoclonal

Recombinant IgM Antibody IGHM/3776R / Recombinant Rabbit Monoclonal [clone IGHM/3776R] (V8051)

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  Catalog No Formulation Size Price (USD)  
Image V8051-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 559
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V8051-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 259
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V8051SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 559
Microvalidated Recrabbitmono
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Recombinant IgM Antibody IGHM/3776R Spleen Tissue Immunohistochemistry. IHC staining of FFPE human spleen with recombinant IgM antibody (clone IGHM/3776R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
IHC staining of FFPE human spleen with recombinant IgM antibody (clone IGHM/3776R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant IgM antibody (clone IGHM/3776R) as confirmation of integrity and purity.
Recombinant IgM Antibody IGHM/3776R in Human Serum WB. Western blot testing of human serum with Recombinant IgM Antibody clone IGHM/3776R detects a discrete band at approximately 75 kDa, consistent with the expected size of the immunoglobulin mu heavy chain. Minimal additional reactivity is visible under the tested conditions, producing a clean Western blot signal. This recombinant rabbit monoclonal recognizes the heavy chain component of human IgM.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Host Rabbit
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name IGHM/3776R
Purity Protein A affinity chromatography
UniProt P01871
Localization Cytoplasmic, cell surface, secreted
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Western Blot : 2-4ug/ml
Limitations This Recombinant IgM Antibody IGHM/3776R is available for research use only.
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Description

Recombinant IgM Antibody IGHM/3776R recognizes a protein of 75kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin s lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

For detection of total human IgM, see our Human IgM ELISA Antibody RM121 page.

Application Notes

Optimal dilution of the Recombinant IgM Antibody IGHM/3776R should be determined by the researcher.

Immunogen

Full length recombinant human IGHM protein was used as the immunogen for the Recombinant IgM Antibody IGHM/3776R.

Storage

Store the Recombinant IgM Antibody IGHM/3776Ry at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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