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Home >> Antibodies >> Recombinant IgG Antibody / Rabbit Monoclonal

Recombinant IgG Antibody / Rabbit Monoclonal [clone GHC07-2R] (V3710)

  Catalog No Formulation Size Price (USD)  
Image V3710-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3710-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3710SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V3710IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
Microvalidated Recrabbitmono
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IHC staining of FFPE human tonsil tissue with recombinant IgG antibody (clone GHC07-2R). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.
Availability 1-3 business days
Species Reactivity Human. Other species not tested.
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name GHC07-2R
Purity Protein A affinity chromatography
UniProt P01857
Localization Cytoplasm, cell surface and secreted
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Prediluted IHC only format : incubate for 30 min at RT (1)
Limitations This recombinant IgG antibody is available for research use only.
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Description

This antibody reacts with all sub-classes of gamma chain of human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. It is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant IgG antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Human Ig Gamma Chain was used as the immunogen for this recombinant IgG antibody.

Storage

Store the recombinant IgG antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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