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Home >> Antibodies >> Recombinant ICAM3 Antibody

Recombinant ICAM3 Antibody [clone ICAM3/2873R] (V7507)

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  Catalog No Formulation Size Price (USD)  
Image V7507-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7507-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7507SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V7507IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
Microvalidated Recrabbitmono
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IHC staining of FFPE human tonsil tissue with recombinant ICAM3 antibody (clone ICAM3/2873R). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
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IHC staining of FFPE human tonsil tissue with recombinant ICAM3 antibody (clone ICAM3/2873R). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE analysis of purified, BSA-free recombinant ICAM3 antibody (clone ICAM3/2873R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name ICAM3/2873R
Purity Protein A affinity chromatography
UniProt P32942
Localization Membrane, cytoplasm
Applications Blocks binding of CD11a (LFA-1) to CD50 (ICAM-3) (order BSA/sodium azide-free format) :
Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT
Limitations This recombinant ICAM3 antibody is available for research use only.
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Description

Recognizes an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C.

Application Notes

Optimal dilution of the recombinant ICAM3 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Full length recombinant human protein was used as the immunogen for the recombinant ICAM3 antibody.

Storage

Store the recombinant ICAM3 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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