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Home >> Antibodies >> Recombinant IBA1 Antibody / AIF1

Recombinant IBA1 Antibody / AIF1 [clone rAIF1/1909] (V3831)

  Catalog No Formulation Size Price (USD)  
Image V3831-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3831-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3831SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recmousemono
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IHC testing of FFPE human lymph node with recombinant IBA1 antibody (clone rAIF1/1909). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human tonsil with recombinant IBA1 antibody (clone rAIF1/1909). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using recombinant IBA1 antibody (clone rAIF1/1909). These results demonstrate the foremost specificity of the rAIF1/1909 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free recombinant IBA1 antibody (clone rAIF1/1909) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rAIF1/1909
Purity Protein G affinity chromatography
UniProt P55008
Localization Cytoplasmic, membranous
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This recombinant IBA1 antibody is available for research use only.
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Description

AIF1 (Allograft inflammatory factor 1), also called IBA1 (Ionized calcium-binding adapter molecule 1) is a cytoplasmic, calcium-binding protein that is thought to play a role in macrophage activation and function. AIF1/IBA1, containing two EF domains, is induced by cytokines and Interferons. In an unstimulated state, it colocalizes with actin, and upon stimulation, translocates to lamellipodia. It is also a marker of human microglia and is expressed by macrophages in injured skeletal muscle. The gene encoding the protein resides in the tumor necrosis factor (TNF) cluster of genes, located in the region represented by the human major histocompatibility complex (MHC).

Application Notes

Titering of the recombinant IBA1 antibody may be required for optimal performance.

Immunogen

A portion of amino acids 1-146 from the human protein was used as the immunogen for the recombinant IBA1 antibody.

Storage

Store the recombinant IBA1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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