Human Lambda Light Chain Antibody for ELISA / Anti-Human Lambda ELISA Antibody [clone RM127] (R20179)

Human Lambda Light Chain Antibody for IHC Human Tonsil Tissue Staining. Immunohistochemistry analysis of Human Lambda Light Chain expression in FFPE human tonsil tissue using Human Lambda Light Chain Antibody clone RM127 demonstrates strong cytoplasmic HRP-DAB brown staining in scattered plasma cells within lymphoid regions, consistent with lambda light chain expression in antibody-producing cells. Surrounding lymphocytes and stromal elements show minimal to no staining. Heat-induced epitope retrieval was performed using either pH 6 citrate buffer or pH 9 Tris-EDTA buffer, supporting reliable detection in FFPE tissue sections.

Human Lambda Light Chain Antibody for ELISA Human Immunoglobulin Lambda Specificity Analysis. ELISA analysis using Human Lambda Light Chain Antibody for ELISA clone RM127 demonstrates selective reactivity to lambda light chains across multiple human immunoglobulin classes, including IgG subclasses, IgM, and IgA. No cross-reactivity is observed with kappa light chains or with mouse, rat, or goat IgG, confirming strong specificity for human lambda light chain detection. These results support reliable use of this antibody for ELISA-based quantification of lambda-containing immunoglobulins.

Human Lambda Light Chain Antibody for ELISA Human IgG3 Lambda Titration Curve. ELISA titration analysis using Human Lambda Light Chain Antibody for ELISA clone RM127 demonstrates concentration-dependent detection of human IgG3 lambda-coated wells. Plates were coated with varying amounts of human IgG3 lambda, followed by incubation with serial dilutions of the antibody and detection using an alkaline phosphatase-conjugated anti-rabbit IgG secondary antibody. The resulting signal shows a clear dose-response relationship, supporting reliable ELISA-based detection of lambda light chain-containing immunoglobulins.