Human Kappa Light Chain Antibody for ELISA / Anti-Kappa Chain ELISA Detection Antibody [clone RM126] (R20178)

Human Kappa Light Chain Antibody Human Tonsil IHC. Immunohistochemistry analysis of Human kappa light chain / IGKC expression in FFPE human tonsil tissue using clone RM126 shows strong cytoplasmic and membranous staining in scattered plasma cells and B lymphocytes within lymphoid follicles, with surrounding non-lymphoid cells largely negative. The staining pattern highlights immunoglobulin-producing cells and is consistent with kappa light chain expression in antibody-secreting populations. Heat-induced epitope retrieval was performed using either pH6 citrate buffer or pH9 Tris-EDTA buffer prior to antibody incubation, supporting reliable detection in formalin-fixed paraffin-embedded tissue.

Human Kappa Light Chain Antibody for ELISA Specificity Analysis. ELISA analysis of recombinant human immunoglobulins demonstrates that clone RM126 selectively recognizes kappa light chain-containing antibodies, with strong signal observed across human IgG subclasses and IgM, while no cross-reactivity is detected with lambda light chain immunoglobulins or non-human IgG from mouse, rat, or goat. The results confirm high specificity for IGKC and support use of this antibody as a reliable detection reagent for kappa light chain measurement in immunoassay formats.

Human Kappa Light Chain Antibody for ELISA Titration Curve. ELISA titration analysis using plates coated with serial dilutions of human IgG4 kappa (50uL per well) demonstrates strong, concentration-dependent binding of clone RM126 across a wide dynamic range. Serial dilution of the primary antibody shows proportional signal reduction with decreasing antibody concentration, confirming high sensitivity and robust binding kinetics. Detection was performed using an alkaline phosphatase-conjugated anti-rabbit IgG secondary antibody, supporting reliable quantification of kappa light chain-containing immunoglobulins in ELISA-based assays.