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Home >> Antibodies >> Recombinant Human Kappa Light Chain Antibody

Recombinant Human Kappa Light Chain Antibody [clone RM126] (R20178)

  Catalog No Formulation Size Price (USD)  
Image R20178-100UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ug 379
Image
R20178SAF-250UG 1 mg/ml in PBS without glycerol/BSA/sodium azide 250 ug 899
IHC testing of FFPE human tonsil tissue with recombinant Human Kappa Light Chain antibody. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.
ELISA of human immunoglobulins shows the recombinant Human Kappa Light Chain antibody reacts to the kappa light chain of hIgs. No cross reactivity with the lambda light chain, mouse/rat/goat IgG.
ELISA Titration: the plate was coated with different amounts of human IgG4κ. A serial dilution of the recombinant Human Kappa Light Chain antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM126
Purity Protein A purified from animal origin-free supernatant
UniProt P01834
Gene ID 3514
Applications ELISA : 0.05-0.2ug/ml
Immunocytochemistry : 0.5-2ug/ml
Immunohistochemistry : 0.5-2ug/ml
Limitations This recombinant Human Kappa Light Chain antibody is available for research use only.
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Description

This recombinant Human Kappa Light Chain antibody reacts to the kappa light chain of human immunoglobulins. No cross reactivity with lambda or mouse/rat/goat IgG.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Human Kappa Light Chain antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Human IgG was used as the immunogen for this recombinant Human Kappa Light Chain antibody.

Storage

Store the recombinant Human Kappa Light Chain antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

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