Human Kappa Light Chain Antibody for ELISA / Biotinylated Anti-Kappa Chain ELISA Detection Antibody [clone RM126] (R20178BTN)

Human Kappa Light Chain Antibody Biotin ELISA Specificity Analysis.ELISA analysis of recombinant human immunoglobulins demonstrates that the parent clone RM126 antibody selectively recognizes kappa light chain-containing antibodies, with strong signal observed across human IgG subclasses and IgM, while no cross-reactivity is detected with lambda light chain immunoglobulins or non-human IgG from mouse, rat, or goat. These results reflect the intrinsic specificity of clone RM126, which is retained in the biotinylated format of the Human Kappa Light Chain Antibody for ELISA / Biotinylated Anti-Kappa Chain ELISA Detection Antibody.

Human Kappa Light Chain Antibody Biotin ELISA Titration Curve.ELISA titration analysis using plates coated with serial dilutions of human IgG4 kappa (50uL per well) demonstrates strong, concentration-dependent binding of clone RM126 across a broad dynamic range. Serial dilution of the primary antibody shows proportional signal reduction with decreasing antibody concentration, confirming high sensitivity and robust binding kinetics. Detection was performed using an alkaline phosphatase-conjugated anti-rabbit IgG secondary antibody. These results reflect the intrinsic binding performance of the parent clone RM126, which is retained in the biotinylated format of the Human Kappa Light Chain Antibody for ELISA / Biotinylated Anti-Kappa Chain ELISA Detection Antibody.

Human Kappa Light Chain Antibody Biotin Human Tonsil IHC Staining.Immunohistochemistry analysis of Human kappa light chain / IGKC expression in FFPE human tonsil tissue demonstrates prominent cytoplasmic and membranous staining in plasma cells and B lymphocytes within lymphoid follicles, while surrounding stromal and non-lymphoid cells remain largely negative. The staining pattern highlights immunoglobulin-producing cells and is consistent with kappa light chain expression in antibody-secreting populations. Heat-induced epitope retrieval was performed using either pH6 citrate buffer or pH9 Tris-EDTA buffer prior to antibody incubation. These results reflect staining obtained with the parent clone RM126 antibody, and the same target recognition is retained in the biotinylated format of the Human Kappa Light Chain Antibody for ELISA / Biotinylated Anti-Kappa Chain ELISA Detection Antibody.