Human IgM Antibody for ELISA / Anti-IgM Detection Antibody [clone RM121] (R20181)

Description

Human immunoglobulin mu (IGHM) encodes the heavy chain constant region of IgM, the first antibody isotype produced during primary immune responses and a key marker of early immune activation. IgM is predominantly secreted as a pentameric complex, composed of five immunoglobulin units linked by a joining chain, which enables exceptionally high avidity binding to antigen. This multivalent structure allows IgM to efficiently capture targets even at low affinity and strongly activate the classical complement pathway, making it a critical component of the innate-like humoral immune response.

Human IgM Antibody for ELISA / Anti-IgM Detection Antibody is specifically optimized for sensitive and selective detection of IgM in ELISA-based immunoassays. Human IgM antibody, also known as anti-IGHM antibody or IgM immunoglobulin antibody, enables accurate quantification of IgM in serum, plasma, and cell culture samples. This recombinant rabbit monoclonal antibody clone RM121 recognizes human IgM and provides a reliable tool for measuring early-stage antibody responses in biological matrices where IgM levels reflect recent or ongoing immune activation.

In ELISA workflows, IgM detection is particularly valuable in serological assays where it serves as an indicator of acute infection or early immune response prior to class switching to IgG or IgA. The pentameric structure of IgM contributes to strong signal generation, but selective detection is required to avoid background from more abundant immunoglobulin classes. The Human IgM Antibody for ELISA / Anti-IgM Detection Antibody functions effectively in ELISA formats to support precise quantification of IgM with minimal cross-reactivity and high signal fidelity, enabling accurate interpretation of immune status.

Clone RM121 antibody recognizes human IgM, ensuring selective detection without cross-reactivity to other immunoglobulin classes such as IgG, IgA, IgD, or IgE. The recombinant rabbit monoclonal format provides high affinity binding, strong reproducibility, and minimal lot-to-lot variability, supporting consistent ELISA performance across experiments. This antibody is well suited for applications requiring reliable detection of IgM in complex biological samples including serum and plasma.

Detection of IgM is widely applied in immunology research, infectious disease studies, and diagnostic assay development. Because IgM is produced rapidly following antigen exposure and declines as adaptive immune responses mature, its measurement provides critical insight into the timing and progression of immune responses. This antibody supports these applications by enabling sensitive and specific detection of IGHM-containing immunoglobulins in ELISA-based systems requiring high performance, reproducibility, and accurate quantification.

This antibody is part of a broader immunoglobulin detection antibody collection, including reagents for Ig classes and light chains across multiple species and immunoassay formats.

Application Notes

The stated application concentrations are suggested starting points. Titration of the Human IgM Antibody for ELISA / Anti-IgM Detection Antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Immunogen

Human IgM was used as the immunogen for this recombinant Human IgM antibody.

Storage

Store the recombinant Human IgM antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Alternate Names

Anti-IgM ELISA antibody, IGHM detection antibody, Human IgM detection antibody, IgM immunoglobulin antibody, IgM ELISA detection antibody