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Home >> Antibodies >> Recombinant Human IgM Antibody (Biotin Conjugate)

Recombinant Human IgM Antibody (Biotin Conjugate) [clone RM121] (R20181BTN)

  Catalog No Formulation Size Price (USD)  
Image R20181BTN-50UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 50 ug 379
Sandwich ELISA of human IgM using the recombinant Human IgM antibody as the capture (100ng/well), and biotinylated anti-human light chains (κ+λ) antibody RM129 as the detect, followed by an alkaline phosphatase conjugated streptavidin.
Sandwich ELISA of human plasma using the recombinant Human IgM antibody as the capture (100ng/well), and biotinylated anti-human light chains (κ+λ) antibody RM129 as the detect, followed by an alkaline phosphatase conjugated streptavidin.
ELISA of human immunoglobulins shows the recombinant Human IgM antibody reacts only to human IgM. No cross reactivity with Human IgG, IgE, IgD, or IgA.
ELISA Titration: the plate was coated with different amounts of human IgM. A serial dilution of the recombinant Human IgM antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
Availability 1-3 business days
Species Reactivity Human
Format Biotin Conjugate
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM121
Purity Protein A purified from animal origin-free supernatant
UniProt P01871
Gene ID 3507
Applications ELISA : 50ng/well-200ng/well (Capture); 0.05-0.2ug/ml (Detection)
Immunocytochemistry : 0.5-2ug/ml
Immunohistochemistry : 0.5-2ug/ml (1)
Limitations This recombinant Human IgM antibody is available for research use only.
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Description

This recombinant Human IgM antibody reacts to the heavy chain of human IgM. No cross reactivity with human IgG, IgA, IgD, or IgE.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Human IgM antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Immunogen

Human IgM was used as the immunogen for this recombinant Human IgM antibody.

Storage

Store the recombinant Human IgM antibody at -20oC.

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