Human IgM Antibody for ELISA / Biotinylated Anti-IgM Detection Antibody [clone RM121] (R20181BTN)

Description

Human immunoglobulin mu (IGHM) encodes the heavy chain constant region of IgM, an immunoglobulin isotype that serves as the primary antibody produced during early immune responses. IgM is predominantly secreted as a pentameric molecule, enabling high avidity antigen binding and efficient activation of the complement cascade. This structural configuration allows IgM to function as a first-line humoral defense, providing rapid immune protection prior to the development of class-switched antibody responses.

Human IgM Antibody for ELISA / Biotinylated Anti-IgM Detection Antibody is engineered for enhanced sensitivity and selective detection of IgM in ELISA workflows utilizing streptavidin-based signal amplification systems. Human IgM antibody, also referred to as anti-IGHM antibody or IgM immunoglobulin antibody, enables accurate detection of IgM in biological samples and supports analysis of early immune responses. This biotinylated recombinant rabbit monoclonal antibody clone RM121 provides selective recognition of human IgM while enabling amplified signal generation for improved assay performance in low-abundance detection scenarios.

In sandwich ELISA configurations, biotinylated detection antibodies provide increased assay sensitivity and expanded dynamic range through streptavidin-mediated amplification. The Human IgM Antibody for ELISA / Biotinylated Anti-IgM Detection Antibody binds selectively to IgM, allowing accurate detection even at low analyte concentrations while maintaining high specificity. This is particularly important in serological studies, infection monitoring, and immune profiling where IgM serves as an indicator of recent exposure and early immune activation.

Clone RM121 antibody recognizes human IgM, ensuring selective detection without cross-reactivity to other immunoglobulin classes such as IgG, IgA, IgD, or IgE. The recombinant rabbit monoclonal format provides strong affinity, consistent performance, and reproducibility across ELISA platforms. Biotin conjugation enhances assay flexibility by enabling compatibility with streptavidin-based detection systems commonly used in ELISA assays.

Measurement of IgM using biotinylated detection antibodies is widely applied in immunology research, infectious disease studies, and diagnostic assay development. Because IgM is produced early following antigen exposure and reflects acute-phase immune activity, accurate detection provides critical insight into immune status and disease progression. This antibody supports these applications by enabling sensitive and reliable detection of IGHM-containing immunoglobulins in ELISA-based systems requiring enhanced signal amplification, high specificity, and consistent assay performance.

This antibody is part of a broader immunoglobulin detection antibody collection, including reagents for Ig classes and light chains across multiple species and immunoassay formats.

Application Notes

The stated application concentrations are suggested starting points. Titration of the Human IgM Antibody for ELISA / Biotinylated Anti-IgM Detection Antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Immunogen

Human IgM was used as the immunogen for this biotinylated recombinant Human IgM antibody.

Storage

Store the recombinant Human IgM antibody at -20oC.

Alternate Names

Biotin anti-IgM antibody, Biotinylated IGHM antibody, Human IgM biotin ELISA antibody, IgM detection biotin antibody, IgM ELISA antibody