- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Human IgG4 Fc Antibody for ELISA / Anti-IgG4 Fc Detection Antibody [clone RM217] (R20191)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
R20191-100UG | 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide | 100 ug | 439 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Rabbit |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG |
| Clone Name | RM217 |
| Purity | Protein A purified from animal origin-free supernatant |
| UniProt | P01861 |
| Gene ID | 3503 |
| Applications | ELISA : 50ng/well-200ng/well (Capture); 0.05-0.2ug/ml (Detection) |
| Limitations | This Human IgG4 Fc Antibody for ELISA / Anti-IgG4 Fc Detection Antibody is available for research use only. |
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Immunoglobulin G4 (IgG4) is a distinct subclass of IgG characterized by reduced effector function and a specialized role in immune regulation and tolerance. Unlike other IgG subclasses, IgG4 exhibits minimal complement activation and limited interaction with Fc receptors, making it functionally associated with chronic antigen exposure, immune modulation, and antibody responses observed in allergy and therapeutic antibody settings. IgG4 is therefore frequently studied in the context of immune tolerance, long-term antigen exposure, and biologic drug monitoring.
IGG4 antibody, also referred to as Immunoglobulin G4 antibody and IgG4 immunoglobulin antibody in the literature, is widely used for subclass-specific detection of IgG4 in immunoassays. Human IgG4 Fc Antibody for ELISA / Anti-IgG4 Fc Detection Antibody is specifically optimized for selective recognition of the Fc region of IgG4, providing consistent and reliable detection independent of antigen-binding variability. Fc-directed detection is particularly advantageous in ELISA workflows, as it enables uniform recognition of IgG4 molecules regardless of Fab specificity, making it well suited for antibody quantification and assay standardization.
This recombinant rabbit monoclonal antibody, clone RM217 antibody, recognizes the Fc region of human IgG4 with high specificity and does not cross react with other human IgG subclasses including IgG1, IgG2, or IgG3. In addition, no cross reactivity is observed with other immunoglobulin classes such as IgM, IgA, IgD, or IgE, nor with IgG from other species including mouse, rat, or goat. This strict specificity profile supports accurate IgG4 detection in complex biological samples and multi-species experimental systems, minimizing background signal and improving assay precision.
In ELISA workflows, Fc-specific IgG4 detection antibodies are commonly used for quantification of total IgG4, detection of therapeutic antibodies, and measurement of subclass-specific immune responses. Because the Fc region is conserved within IgG4 molecules, detection is consistent across different antibody populations, enabling reliable signal generation and reproducibility across experiments. The Human IgG4 Fc Antibody for ELISA / Anti-IgG4 Fc Detection Antibody is well suited for use in direct, indirect, and sandwich ELISA formats requiring high specificity and sensitivity.
Detection of IgG4 is widely applied in immunology research, allergy studies, and therapeutic antibody development. IgG4 is often associated with regulatory immune responses and long-term antigen exposure, and its accurate measurement provides important insight into antibody subclass distribution, immune tolerance, and biologic drug responses. This antibody supports these applications by enabling sensitive and specific detection of the IgG4 Fc region in ELISA-based systems requiring precise subclass discrimination, low background, and consistent performance.
This antibody is part of a broader immunoglobulin detection antibody collection, including reagents for Ig classes and light chains across multiple species and immunoassay formats.
The stated application concentrations are suggested starting points. Titration of the Human IgG4 Fc Antibody for ELISA / Anti-IgG4 Fc Detection Antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Peptide corresponding to the Fc region of hIgG4 was used as the immunogen for this recombinant Human IgG4 Fc antibody.
Store the recombinant Human IgG4 Fc antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
Anti-IgG4 Fc antibody, IgG4 Fc detection antibody, Human IgG4 Fc antibody, IgG4 constant region antibody, Fc-specific IgG4 antibody
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