- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Human IgG4 Fc Antibody for ELISA / Biotinylated Anti-IgG4 Fc Detection Antibody [clone RM217] (R20191BTN)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
R20191BTN-50UG | 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide | 50 ug | 439 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Biotin Conjugate |
| Host | Rabbit |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG |
| Clone Name | RM217 |
| Purity | Protein A purified from animal origin-free supernatant |
| UniProt | P01861 |
| Gene ID | 3503 |
| Applications | ELISA : 50ng/well-200ng/well (Capture); 0.05-0.2ug/ml (Detection) |
| Limitations | This Human IgG4 Fc Antibody for ELISA / Biotinylated Anti-IgG4 Fc Detection Antibody is available for research use only. |
|
Review this product on BioCompare and get a $20 Amazon gift card
|
Related Products
|
Immunoglobulin G4 (IgG4) is a distinct subclass of IgG characterized by reduced effector function and a specialized role in immune regulation and tolerance. Unlike other IgG subclasses, IgG4 exhibits minimal complement activation and limited interaction with Fc receptors, and can undergo Fab-arm exchange, resulting in functionally monovalent antibodies. These properties make IgG4 closely associated with chronic antigen exposure, immune modulation, allergy, and therapeutic antibody responses. As a result, accurate detection of IgG4 is critical in studies of immune tolerance, biologic drug monitoring, and long-term immune dynamics.
IGG4 antibody, also referred to as Immunoglobulin G4 antibody and IgG4 immunoglobulin antibody in the literature, is widely used for subclass-specific detection of IgG4 in immunoassays. Human IgG4 Fc Antibody for ELISA / Biotinylated Anti-IgG4 Fc Detection Antibody is specifically optimized for selective recognition of the Fc region of IgG4 while enabling enhanced signal amplification through biotin-streptavidin detection systems. Fc-directed detection ensures consistent recognition across IgG4 molecules regardless of antigen-binding variability, making it particularly advantageous for quantitative ELISA workflows and assay standardization.
This recombinant rabbit monoclonal antibody, clone RM217 antibody, recognizes the Fc region of human IgG4 with high specificity and does not cross react with other human IgG subclasses including IgG1, IgG2, or IgG3. In addition, no cross reactivity is observed with other immunoglobulin classes such as IgM, IgA, IgD, or IgE, nor with IgG from other species including mouse, rat, or goat. This strict specificity profile supports accurate IgG4 detection in complex biological samples and multi-species experimental systems, minimizing background signal and improving assay precision.
In ELISA workflows, biotinylated Fc-specific IgG4 detection antibodies are commonly used in sandwich ELISA and high-sensitivity detection formats where signal amplification is required. Biotin conjugation enables strong interaction with streptavidin-linked enzymes, increasing assay sensitivity and expanding dynamic range, particularly in low-abundance or complex samples such as serum or plasma. Because the Fc region is conserved within IgG4 molecules, detection remains uniform across antibody populations, supporting reproducible and reliable signal generation.
Detection of IgG4 is widely applied in immunology research, allergy studies, and therapeutic antibody development. IgG4 is frequently associated with regulatory immune responses and long-term antigen exposure, and its accurate measurement provides important insight into antibody subclass distribution, immune tolerance, and biologic drug responses. This antibody supports these applications by enabling sensitive and specific detection of the IgG4 Fc region in ELISA-based systems requiring enhanced signal amplification, low background, and consistent performance.
This antibody is part of a broader immunoglobulin detection antibody collection, including reagents for Ig classes and light chains across multiple species and immunoassay formats.
The stated application concentrations are suggested starting points. Titration of the Human IgG4 Fc Antibody for ELISA / Biotinylated Anti-IgG4 Fc Detection Antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Peptide corresponding to the Fc region of human IgG4 was used as the immunogen for this biotinylated recombinant Human IgG4 Fc antibody.
Store the recombinant Human IgG4 Fc antibody at -20oC.
Biotin anti-IgG4 Fc antibody, IgG4 Fc detection antibody, Human IgG4 Fc antibody, IgG4 constant region antibody, Fc-specific IgG4 antibody
Your bulk quote request has been submitted successfully!
Please contact us if you have any questions.
Powered by Bioz
