Human IgG4 Antibody for ELISA / Hinge Region IgG4 Detection Antibody [clone RM120] (R20190)

Description

Human immunoglobulin gamma 4 (IGHG4) encodes the heavy chain constant region of IgG4, a subclass distinguished by its unique hinge region structure and reduced effector function compared to other IgG subclasses. Unlike IgG1, IgG2, and IgG3, IgG4 exhibits limited complement activation and weaker Fc receptor binding, and is best known for undergoing Fab arm exchange, a process in which half-molecules swap to generate functionally monovalent antibodies. The hinge region is central to this process, enabling structural flexibility and interchain exchange that define IgG4 behavior in both physiological and engineered antibody systems.

Human IgG4 Antibody for ELISA / Hinge Region IgG4 Detection Antibody is specifically optimized for detection of IgG4 through recognition of the hinge region rather than the Fc domain. Human IgG4 antibody, also known as anti-IGHG4 antibody or hinge-specific IgG4 antibody, enables selective detection of structural features associated with IgG4 flexibility, Fab arm exchange, and conformational dynamics. This recombinant rabbit monoclonal antibody clone RM120 recognizes a peptide corresponding to the hinge region of human IgG4, providing a structurally focused detection approach that complements traditional Fc-directed antibodies.

In ELISA workflows, hinge-directed detection provides a distinct advantage by targeting a functionally relevant and structurally dynamic region of IgG4. The Human IgG4 Antibody for ELISA / Hinge Region IgG4 Detection Antibody functions effectively in ELISA-based formats to detect IgG4 while offering additional insight into hinge-associated structural integrity. This is particularly valuable in applications involving antibody stability assessment, engineered antibody formats, and therapeutic antibody characterization, where hinge-mediated flexibility influences antibody performance.

Clone RM120 antibody recognizes the hinge region of human IgG4, ensuring selective detection without cross-reactivity to other IgG subclasses including IgG1, IgG2, and IgG3, or to other immunoglobulin classes such as IgM, IgA, IgD, and IgE. The recombinant rabbit monoclonal format provides high affinity binding, strong reproducibility, and minimal lot-to-lot variability, supporting consistent ELISA performance across experiments.

Detection of IgG4 hinge structure is widely applied in immunology research, antibody engineering, and therapeutic antibody development, where Fab arm exchange and structural flexibility influence antibody function. Because IgG4 exhibits distinct structural and functional properties compared to other subclasses, hinge-specific detection provides valuable insight into antibody conformation, stability, and biological activity. This antibody supports these applications by enabling selective detection of IGHG4-containing immunoglobulins in ELISA-based systems requiring high specificity, structural relevance, and consistent performance.

This antibody is part of a broader immunoglobulin detection antibody collection, including reagents for Ig classes and light chains across multiple species and immunoassay formats.

Application Notes

The stated application concentrations are suggested starting points. Titration of the Human IgG4 Antibody for ELISA / Hinge Region IgG4 Detection Antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Immunogen

Peptide corresponding to the hinge region of human IgG4 was used as the immunogen for this recombinant Human IgG4 antibody.

Storage

Store the recombinant Human IgG4 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Alternate Names

IgG4 hinge region antibody, IGHG4 hinge antibody, Human IgG4 hinge detection antibody, IgG4 structural antibody, IgG4 hinge ELISA antibody