Human IgG4 Antibody for ELISA / Biotinylated Hinge Region IgG4 Detection Antibody [clone RM120] (R20190BTN)

Description

Human immunoglobulin gamma 4 (IGHG4) encodes the heavy chain constant region of IgG4, a subclass defined by its structurally distinct hinge region and reduced effector function relative to other IgG subclasses. IgG4 is unique in its ability to undergo Fab arm exchange, resulting in antibodies with altered valency and antigen-binding behavior. The hinge region is critical to this process, enabling molecular flexibility and interchain exchange that distinguish IgG4 from IgG1, IgG2, and IgG3 and contribute to its specialized biological role.

Human IgG4 Antibody for ELISA / Biotinylated Hinge Region IgG4 Detection Antibody is engineered for enhanced sensitivity and hinge-specific detection in ELISA workflows utilizing streptavidin-based signal amplification systems. Human IgG4 antibody, also referred to as anti-IGHG4 antibody or hinge-specific IgG4 antibody, enables selective detection of IgG4 structural features associated with hinge-mediated flexibility and conformational dynamics. This biotinylated recombinant rabbit monoclonal antibody clone RM120 recognizes a peptide corresponding to the hinge region of human IgG4, providing a targeted detection strategy distinct from Fc-directed approaches.

In sandwich ELISA configurations, biotinylated detection antibodies provide increased assay sensitivity and expanded dynamic range through streptavidin-mediated amplification. The Human IgG4 Antibody for ELISA / Biotinylated Hinge Region IgG4 Detection Antibody binds selectively to IgG4, allowing accurate detection even at low analyte concentrations while maintaining hinge-region specificity. This is particularly useful in studies involving antibody engineering, conformational analysis, and therapeutic antibody development where hinge integrity influences function.

Clone RM120 antibody targets the hinge region of human IgG4, ensuring selective detection without cross-reactivity to other IgG subclasses including IgG1, IgG2, and IgG3, or to other immunoglobulin classes such as IgM, IgA, IgD, and IgE. The recombinant rabbit monoclonal format provides strong affinity, consistent performance, and reproducibility across ELISA platforms. Biotin conjugation enhances assay flexibility by enabling compatibility with streptavidin-based detection systems commonly used in ELISA assays.

Measurement of IgG4 hinge structure is widely applied in immunology research, antibody engineering, and therapeutic antibody development, where structural flexibility and Fab arm exchange influence antibody behavior. Because IgG4 exhibits distinct conformational properties compared to other subclasses, hinge-specific detection provides important insight into antibody stability, structure, and function. This antibody supports these applications by enabling sensitive and selective detection of IGHG4-containing immunoglobulins in ELISA-based systems requiring enhanced signal amplification, high specificity, and consistent assay performance.

This antibody is part of a broader immunoglobulin detection antibody collection, including reagents for Ig classes and light chains across multiple species and immunoassay formats.

Application Notes

The stated application concentrations are suggested starting points. Titration of the Human IgG4 Antibody for ELISA / Biotinylated Hinge Region IgG4 Detection Antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Immunogen

Peptide corresponding to the hinge region of human IgG4 was used as the immunogen for this recombinant Human IgG4 antibody.

Storage

Store the recombinant Human IgG4 antibody at -20oC.

Alternate Names

Biotin IgG4 hinge antibody, Biotinylated IGHG4 hinge antibody, Human IgG4 hinge biotin antibody, IgG4 structural biotin antibody, IgG4 hinge ELISA antibody