Human IgG3 Antibody for ELISA / Anti-Human IgG3 ELISA Detection Antibody [clone RM119] (R20189)

Human IgG3 Antibody for ELISA Sandwich ELISA Human Plasma. Sandwich ELISA using human plasma demonstrates that clone RM119 functions effectively as a capture antibody for Human IgG3 / IGHG3, with signal intensity decreasing proportionally with sample dilution, indicating robust and concentration-dependent detection. Captured IgG3 was detected using a biotinylated anti-human light chains (kappa + lambda) antibody (clone RM129), followed by alkaline phosphatase-conjugated streptavidin for signal development. The assay configuration supports reliable quantification of IgG3 in complex biological samples and highlights the utility of this Human IgG3 Antibody for ELISA / Anti-Human IgG3 ELISA Detection Antibody in sandwich ELISA-based immunoglobulin measurement.

Human IgG3 Antibody for ELISA Sandwich ELISA Human IgG3. Sandwich ELISA using purified human IgG3 demonstrates that clone RM119 functions effectively as a capture antibody for Human IgG3 / IGHG3, with signal intensity decreasing proportionally with antigen concentration, indicating strong and concentration-dependent detection. Captured IgG3 was detected using a biotinylated anti-human light chains (kappa + lambda) antibody (clone RM129), followed by alkaline phosphatase-conjugated streptavidin for signal development. This assay configuration supports accurate quantification of IgG3 and highlights the utility of this Human IgG3 Antibody for ELISA / Anti-Human IgG3 ELISA Detection Antibody in sandwich ELISA-based immunoassays.

Human IgG3 Antibody Immunohistochemistry Human Lymphoid Tissue. Immunohistochemistry analysis of FFPE human lymphoid tissue using Human IgG3 Antibody / Anti-Human IgG3 Detection Antibody (clone RM119) demonstrates scattered cytoplasmic staining in lymphoid cells consistent with immunoglobulin-producing cell populations, while surrounding cells remain largely negative. This staining pattern reflects detection of IgG3 / IGHG3 within antibody-secreting cells and supports the use of this antibody for identifying IgG3-expressing cell populations in tissue sections. Heat-induced epitope retrieval was performed using either pH6 citrate buffer or pH9 Tris-EDTA buffer prior to antibody incubation.

Human IgG3 Antibody for ELISA Subclass Specificity Analysis. ELISA analysis of human immunoglobulins demonstrates that clone RM119 selectively recognizes Human IgG3, with strong signal observed across tested concentrations. No cross-reactivity is detected with IgG1, IgG2, or IgG4, or with other immunoglobulin classes including IgM, IgA, IgD, and IgE, or with mouse, rat, or goat IgG. The binding profile is consistent with heavy chain-directed recognition and supports highly specific detection of IgG3 in ELISA-based immunoassays. These results support the use of this Human IgG3 Antibody for ELISA / Anti-Human IgG3 ELISA Detection Antibody for precise subclass-specific detection.

Human IgG3 Antibody for ELISA Titration Curve. ELISA titration analysis using plates coated with serial dilutions of human IgG3 demonstrates strong, concentration-dependent binding of clone RM119 across a broad dynamic range. Signal intensity decreases proportionally with antibody dilution, confirming high sensitivity and consistent binding performance. Detection was performed using an alkaline phosphatase-conjugated anti-rabbit IgG secondary antibody, supporting reliable quantification of Human IgG3 in ELISA-based immunoassays.