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Home >> Antibodies >> Recombinant Human IgG3 Antibody

Recombinant Human IgG3 Antibody [clone RM119] (R20189)

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  Catalog No Formulation Size Price (USD)  
Image R20189-100UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ug 419
Microvalidated Recrabbitmono
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Sandwich ELISA with human plasma using recombinant Human IgG3 antibody as the capture, and biotinylated anti-human light chains (κ+λ) antibody RM129 as the detect, followed by an AP conjugated streptavidin.
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Sandwich ELISA with human plasma using recombinant Human IgG3 antibody as the capture, and biotinylated anti-human light chains (κ+λ) antibody RM129 as the detect, followed by an AP conjugated streptavidin.
Sandwich ELISA with human IgG3 using recombinant Human IgG3 antibody as the capture, and biotinylated anti-human light chains (κ+λ) antibody RM129 as the detect, followed by an AP conjugated streptavidin.
IHC testing of FFPE human lymphoid tissue with recombinant Human IgG3 antibody. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.
ELISA of human immunoglobulins shows recombinant Human IgG3 antibody reacts only to hIgG3. No cross reactivity with IgG1, IgG2, IgG4, IgE, IgD, IgA, mouse/rat/goat IgG.
ELISA Titration: the plate was coated with different amounts of hIgG3. A serial dilution of recombinant Human IgG3 antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM119
Purity Protein A purified from animal origin-free supernatant
UniProt P01860
Gene ID 3502
Applications ELISA : 50ng/well-200ng/well (Capture); 0.05-0.2ug/ml (Detection)
Immunocytochemistry : 0.5-2ug/ml
Immunohistochemistry : 0.5-2ug/ml
Limitations This recombinant Human IgG3 antibody is available for research use only.
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Description

This recombinant Human IgG3 antibody reacts to the heavy chain of hIgG3. No cross reactivity with IgG1, IgG2, IgG4, IgM, IgA, IgD, IgE, mouse/rat/goat IgG.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Human IgG3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Human IgG3 was used as the immunogen for this recombinant Human IgG3 antibody.

Storage

Store the recombinant Human IgG3 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

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