Human IgG3 Antibody for ELISA / Biotinylated Anti-Human IgG3 ELISA Detection Antibody [clone RM119] (R20189BTN)

Human IgG3 Antibody Biotin Sandwich ELISA using Human IgG3. Sandwich ELISA using purified human IgG3 demonstrates that the parent clone RM119 antibody functions effectively as a capture antibody for Human IgG3 / IGHG3, with signal intensity decreasing proportionally with antigen concentration, indicating strong and concentration-dependent detection. Captured IgG3 was detected using a biotinylated anti-human light chains (kappa + lambda) antibody (clone RM129), followed by alkaline phosphatase-conjugated streptavidin for signal development. These results reflect the intrinsic binding performance of clone RM119 and are representative of the characteristics retained in the biotinylated format of the Human IgG3 Antibody for ELISA / Biotinylated Anti-Human IgG3 ELISA Detection Antibody.

Human IgG3 Antibody Biotin Sandwich ELISA with Human Plasma. Sandwich ELISA using human plasma demonstrates that the parent clone RM119 antibody functions effectively as a capture antibody for Human IgG3 / IGHG3, with signal intensity decreasing proportionally with sample dilution, indicating robust and concentration-dependent detection. Captured IgG3 was detected using a biotinylated anti-human light chains (kappa + lambda) antibody (clone RM129), followed by alkaline phosphatase-conjugated streptavidin for signal development. These results reflect the intrinsic binding performance of clone RM119 and are representative of the characteristics retained in the biotinylated format of the Human IgG3 Antibody for ELISA / Biotinylated Anti-Human IgG3 ELISA Detection Antibody.

Human IgG3 Antibody Biotin IHC Human Lymphoid Tissue. Immunohistochemistry analysis of FFPE human lymphoid tissue demonstrates that the parent clone RM119 antibody produces scattered cytoplasmic staining in lymphoid cells consistent with immunoglobulin-producing cell populations, while surrounding cells remain largely negative. This staining pattern reflects detection of IgG3 / IGHG3 within antibody-secreting cells and is representative of the performance retained in the biotinylated format of the Human IgG3 Antibody / Biotinylated Anti-Human IgG3 Detection Antibody. Heat-induced epitope retrieval was performed using either pH6 citrate buffer or pH9 Tris-EDTA buffer prior to antibody incubation.

Human IgG3 Antibody Biotin ELISA Subclass Specificity Analysis. ELISA analysis of human immunoglobulins demonstrates that the parent clone RM119 antibody selectively recognizes Human IgG3, with strong signal observed across tested concentrations. No cross-reactivity is detected with IgG1, IgG2, or IgG4, or with other immunoglobulin classes including IgM, IgA, IgD, and IgE, or with mouse, rat, or goat IgG. This binding profile reflects heavy chain-directed specificity for Human IgG3 and is retained in the biotinylated format of the Human IgG3 Antibody for ELISA / Biotinylated Anti-Human IgG3 ELISA Detection Antibody.

Human IgG3 Antibody Biotin ELISA Titration Curve. ELISA titration using plates coated with serial dilutions of human IgG3 demonstrates strong, concentration-dependent binding of clone RM119 across a broad dynamic range. Signal intensity decreases proportionally with antibody dilution, confirming high sensitivity and consistent binding kinetics. Detection was performed using an alkaline phosphatase-conjugated anti-rabbit IgG secondary antibody. This titration profile reflects the intrinsic binding performance of the parent clone RM119 antibody and is representative of the characteristics retained in the biotinylated format of the Human IgG3 Antibody for ELISA / Biotinylated Anti-Human IgG3 ELISA Detection Antibody.