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Home >> Antibodies >> Recombinant Human IgG2 Antibody

Recombinant Human IgG2 Antibody [clone RM118] (R20188)

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Image R20188-100UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ug 419
Microvalidated Recrabbitmono
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Sandwich ELISA with human plasma using recombinant Human IgG2 antibody as the capture, and biotinylated anti-human light chains (κ+λ) antibody RM129 as the detect, followed by an AP conjugated streptavidin.
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Sandwich ELISA with human plasma using recombinant Human IgG2 antibody as the capture, and biotinylated anti-human light chains (κ+λ) antibody RM129 as the detect, followed by an AP conjugated streptavidin.
Sandwich ELISA with human IgG2 using recombinant Human IgG2 antibody as the capture, and biotinylated anti-human light chains (κ+λ) antibody RM129 as the detect, followed by an AP conjugated streptavidin.
ELISA of human immunoglobulins shows recombinant Human IgG2 antibody only reacted to hIgG2. No cross reactivity with IgG1, IgG3, IgG4, IgE, IgD, IgA, mouse/rat/goat IgG.
ELISA Titration: the plate was coated with different amounts of human IgG2. A serial dilution of recombinant Human IgG2 antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM118
Purity Protein A purified from animal origin-free supernatant
UniProt P01859
Gene ID 3501
Applications ELISA : 50ng/well-200ng/well (Capture); 0.05-0.2ug/ml (Detection)
Limitations This recombinant Human IgG2 antibody is available for research use only.
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Description

This recombinant Human IgG2 antibody reacts to the heavy chain of human IgG2. No cross reactivity with IgG1, IgG3, IgG4, IgM, IgA, IgD, IgE, mouse/rat/goat IgG.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Human IgG2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Human IgG2 was used as the immunogen for this recombinant Human IgG2 antibody.

Storage

Store the recombinant Human IgG2 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

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