Recombinant Human IgG Antibody rIG266 / Microarray Validated [clone rIG266] (V3583)

Recombinant Human IgG Antibody rIG266 Tonsil Immunohistochemistry. IHC testing of FFPE human tonsil with recombinant human IgG antibody clone rIG266. Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE analysis of purified, BSA-free recombinant human IgG antibody (clone rIG266) as confirmation of integrity and purity.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using recombinant Human IgG antibody (clone rIG266). These results demonstrate the foremost specificity of the rIG266 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Recombinant Human IgG Antibody rIG266 Human Serum WB. Western blot analysis was performed using Recombinant Human IgG Antibody rIG266 on human serum lysate. A distinct immunoreactive band is detected at approximately 50–55 kDa, consistent with the expected molecular weight of the human IgG heavy chain. The blot exhibits a strong, specific signal with minimal background, supporting reliable detection of endogenous human IgG in serum samples. These results demonstrate that Recombinant Human IgG Antibody rIG266 is suitable for Western blot detection of human IgG in serum.