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Home >> Antibodies >> Recombinant Human IgG Antibody (Biotin Conjugate)

Recombinant Human IgG Antibody (Biotin Conjugate) [clone RM116] (R20177BTN)

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  Catalog No Formulation Size Price (USD)  
Image R20177BTN-50UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 50 ug 419
Microvalidated Recrabbitmono
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Sandwich ELISA of human IgG using the recombinant Human IgG antibody (clone RM116) as the capture (100ng/well), and biotinylated human light chains (κ+ λ) antibody (clone RM129) as the detect (1ug/mL), followed by an alkaline phosphatase conjugated streptavidin.
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Sandwich ELISA of human IgG using the recombinant Human IgG antibody (clone RM116) as the capture (100ng/well), and biotinylated human light chains (κ+ λ) antibody (clone RM129) as the detect (1ug/mL), followed by an alkaline phosphatase conjugated streptavidin.
Sandwich ELISA of human plasma using the recombinant Human IgG antibody (clone RM116) as the capture (100ng/well), and biotinylated human light chains (κ+ λ) antibody (clone RM129) as the detect (1ug/mL), followed by an alkaline phosphatase conjugated streptavidin.
IHC testing of FFPE human tonsil with recombinant Human IgG antibody.
ELISA of human immunoglobulins shows the recombinant Human IgG antibody reacted to the G1, G2, G3, G4 heavy chain of human IgGs, and the Fc of human IgG. No cross reactivity with other human heavy chains, mouse/rat/goat IgG.
ELISA Titration: the plate was coated with different amounts of human IgG1. A serial dilution of the recombinant Human IgG antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
Availability 1-3 business days
Species Reactivity Human
Format Biotin Conjugate
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM116
Purity Protein A purified from animal origin-free supernatant
UniProt P01857 , P01859 , P01860 , P01861
Gene ID 3500 , 3501 , 3502 , 3503
Applications ELISA : 50ng/well-200ng/well (Capture); 0.05-0.2ug/ml (Detection)
Immunocytochemistry : 0.5-2ug/ml
Immunohistochemistry : 0.5-2ug/ml (1)
Limitations This recombinant Human IgG antibody is available for research use only.
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Description

This recombinant Human IgG antibody reacts to the Fc region of all gamma heavy chains of human immunoglobulins, including G1, G2, G3, and G4. No cross reactivity with other human heavy chains, mouse IgG, rat IgG or goat IgG.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Human IgG antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Immunogen

Human IgG was used as the immunogen for this recombinant Human IgG antibody.

Storage

Store the recombinant Human IgG antibody at -20oC.

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