Human IgG Antibody for ELISA / Biotinylated Anti-Human IgG Detection Antibody [clone RM116] (R20177BTN)

Description

Human immunoglobulin gamma (IGHG) encodes the heavy chain constant regions of IgG, the predominant immunoglobulin class in human serum and a key component of adaptive immunity. IgG includes multiple subclasses such as IgG1, IgG2, IgG3, and IgG4, which share conserved structural elements including the Fc region that mediates effector functions and interactions with immune cells. Due to its high abundance and long half-life, IgG serves as a primary marker of sustained immune responses and antibody-mediated immunity.

Human IgG Antibody for ELISA / Biotinylated Anti-Human IgG Detection Antibody is engineered for enhanced sensitivity and broad detection of total IgG in ELISA workflows utilizing streptavidin-based signal amplification systems. Human IgG antibody, also referred to as anti-IGHG antibody or IgG immunoglobulin antibody, enables accurate detection of IgG across subclasses in biological samples including serum, plasma, and cell culture supernatants. This biotinylated recombinant rabbit monoclonal antibody clone RM116 provides selective recognition of human IgG while enabling amplified signal generation for improved assay performance.

In sandwich ELISA configurations, biotinylated detection antibodies provide increased assay sensitivity and expanded dynamic range through streptavidin-mediated amplification. The Human IgG Antibody for ELISA / Biotinylated Anti-Human IgG Detection Antibody binds selectively to IgG, allowing accurate detection across a wide concentration range while maintaining specificity against non-IgG immunoglobulin classes. This is particularly useful in assays involving antibody quantification, immune response monitoring, and therapeutic antibody measurement where reliable signal amplification is required.

Clone RM116 antibody recognizes human IgG, supporting detection across IgG subclasses while maintaining specificity against non-IgG immunoglobulin classes such as IgM, IgA, IgD, and IgE. The recombinant rabbit monoclonal format provides strong affinity, consistent performance, and reproducibility across ELISA platforms. Biotin conjugation enhances assay flexibility by enabling compatibility with streptavidin-based detection systems commonly used in ELISA assays.

Measurement of total IgG using biotinylated detection antibodies is widely applied in immunology research, therapeutic antibody development, and diagnostic assay design. Because IgG reflects established immune responses and antibody production, accurate detection provides critical insight into antibody titer, immune status, and long-term immunity. This antibody supports these applications by enabling sensitive and reliable detection of IGHG-containing immunoglobulins in ELISA-based systems requiring enhanced signal amplification, high specificity, and consistent assay performance.

This antibody is part of a broader immunoglobulin detection antibody collection, including reagents for Ig classes and light chains across multiple species and immunoassay formats.

Application Notes

The stated application concentrations are suggested starting points. Titration of the Human IgG Antibody for ELISA / Biotinylated Anti-Human IgG Detection Antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Immunogen

Human IgG was used as the immunogen for this biotinylated recombinant Human IgG antibody.

Storage

Store the recombinant Human IgG antibody at -20oC.

Alternate Names

Biotin anti-human IgG antibody, Biotinylated IGHG antibody, Human IgG biotin ELISA antibody, IgG detection biotin antibody, pan-IgG antibody