- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Human IgE Antibody for ELISA / Anti-IgE Detection Antibody [clone RM122] (R20186)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
R20186-100UG | 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide | 100 ug | 439 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Rabbit |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG |
| Clone Name | RM122 |
| Purity | Protein A purified from animal origin-free supernatant |
| UniProt | P01854 |
| Gene ID | 3497 |
| Applications | ELISA : 10ng/well-100ng/well (Capture); 0.01-0.1ug/ml (Detection) |
| Limitations | This Human IgE Antibody for ELISA / Anti-IgE Detection Antibody is available for research use only. |
|
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Human immunoglobulin epsilon (IGHE) encodes the heavy chain constant region of IgE, an immunoglobulin class that plays a central role in allergic responses and immediate hypersensitivity reactions. IgE is the least abundant immunoglobulin in circulation, typically present at concentrations several orders of magnitude lower than IgG, yet it exerts potent biological effects through high-affinity binding to Fc epsilon receptors on mast cells and basophils. Cross-linking of receptor-bound IgE by allergen triggers rapid cellular activation and release of inflammatory mediators, making IgE a key biomarker of allergic disease and immune activation.
Human IgE Antibody for ELISA / Anti-IgE Detection Antibody is specifically optimized for sensitive and selective detection of IgE in ELISA-based immunoassays. Human IgE antibody, also known as anti-IGHE antibody or IgE immunoglobulin antibody, enables accurate quantification of IgE in serum, plasma, and cell culture samples where concentrations are typically low. This recombinant rabbit monoclonal antibody clone RM122 recognizes human IgE and provides a reliable tool for detecting low-abundance IgE with high specificity.
In ELISA workflows, detection of IgE requires high analytical sensitivity and minimal background due to the presence of significantly higher levels of other immunoglobulin classes. The Human IgE Antibody for ELISA / Anti-IgE Detection Antibody functions effectively in ELISA formats to support precise quantification of IgE while minimizing cross-reactivity and non-specific signal. This is particularly important in studies of allergy, asthma, and immune profiling, where small changes in IgE concentration can reflect meaningful differences in immune status.
Clone RM122 antibody recognizes human IgE, ensuring selective detection without cross-reactivity to other immunoglobulin classes such as IgG, IgA, IgM, or IgD. The recombinant rabbit monoclonal format provides high affinity binding, strong reproducibility, and minimal lot-to-lot variability, supporting consistent ELISA performance across experiments. This antibody is well suited for applications requiring precise detection of IgE in complex biological matrices including serum and plasma.
Detection of IgE is widely applied in immunology research, allergy studies, and biomarker analysis. Because IgE levels are closely associated with hypersensitivity reactions, atopic disease, and immune activation, accurate measurement provides valuable insight into disease mechanisms and immune dynamics. This antibody supports these applications by enabling reliable detection of IGHE-containing immunoglobulins in ELISA-based systems requiring high sensitivity, specificity, and consistent performance.
This antibody is part of a broader immunoglobulin detection antibody collection, including reagents for Ig classes and light chains across multiple species and immunoassay formats.
The stated application concentrations are suggested starting points. Titration of the Human IgE Antibody for ELISA / Anti-IgE Detection Antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Human IgE was used as the immunogen for this recombinant Human IgE antibody.
Store the recombinant Human IgE antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
Anti-IgE ELISA antibody, IGHE detection antibody, Human IgE detection antibody, IgE immunoglobulin antibody, IgE ELISA detection antibody
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