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Home >> Antibodies >> Recombinant Human IgE Antibody

Recombinant Human IgE Antibody [clone RM122] (R20186)

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  Catalog No Formulation Size Price (USD)  
Image R20186-100UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ug 419
Microvalidated Recrabbitmono
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Sandwich ELISA with human plasma using recombinant Human IgE antibody as the capture (25ng/well) and biotinylated anti-human light chains (κ+λ) antibody RM129 as the detect, followed by an alkaline phosphatase conjugated streptavidin.
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Sandwich ELISA with human plasma using recombinant Human IgE antibody as the capture (25ng/well) and biotinylated anti-human light chains (κ+λ) antibody clone RM129 as the detect, followed by an alkaline phosphatase conjugated streptavidin.
ELISA of hIgs shows recombinant Human IgE antibody reacts only to IgEλ from human myeloma and the IgE from human plasma. No cross reactivity with IgG, IgM, IgD, or IgA.
ELISA Titration: the plate was coated with different amounts of hIgE. A serial dilution of recombinant Human IgE antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM122
Purity Protein A purified from animal origin-free supernatant
UniProt P01854
Gene ID 3497
Applications ELISA : 10ng/well-100ng/well (Capture); 0.01-0.1ug/ml (Detection)
Limitations This recombinant Human IgE antibody is available for research use only.
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Description

This recombinant Human IgE antibody reacts to hIgE. No cross reactivity with IgG, IgM, IgD, or IgA.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Human IgE antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Human IgE was used as the immunogen for this recombinant Human IgE antibody.

Storage

Store the recombinant Human IgE antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

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