Human IgA2 Antibody for ELISA / Biotinylated Anti-IgA2 Detection Antibody [clone RM125] (R20184BTN)

Human IgA2 Antibody Biotin Sandwich ELISA Human IgA2. Sandwich ELISA analysis using purified human IgA2 demonstrates that the parent clone RM125 antibody functions effectively as a capture antibody for Human IgA2 / IGHA2, with signal intensity decreasing proportionally with antigen concentration, indicating strong and concentration-dependent detection. Captured IgA2 was detected using a biotinylated anti-human light chains (kappa + lambda) antibody (clone RM129), followed by alkaline phosphatase-conjugated streptavidin for signal development. These results reflect the intrinsic binding performance of clone RM125 and are representative of the characteristics retained in the biotinylated format of the Human IgA2 Antibody for ELISA / Biotinylated Anti-IgA2 Detection Antibody.

Human IgA2 Antibody Biotin Sandwich ELISA Human Plasma. Sandwich ELISA analysis demonstrates that the parent clone RM125 antibody functions effectively as a capture antibody for Human IgA2 / IGHA2, with signal intensity decreasing proportionally with plasma dilution, indicating strong and concentration-dependent detection. Captured IgA2 was detected using a biotinylated anti-human light chains (kappa + lambda) antibody (clone RM129), followed by alkaline phosphatase-conjugated streptavidin for signal development. This binding profile reflects the intrinsic performance of clone RM125 and is representative of the characteristics retained in the biotinylated format of the Human IgA2 Antibody for ELISA / Biotinylated Anti-IgA2 Detection Antibody.

Human IgA2 Antibody Biotin Immunohistochemistry Human Tonsil Tissue. Immunohistochemistry analysis of FFPE human tonsil tissue demonstrates that the parent clone RM125 antibody produces cytoplasmic staining in plasma cells distributed within interfollicular and subepithelial regions, with minimal background in surrounding lymphoid cells. This staining pattern supports detection of IgA2 / IGHA2 in mucosal-associated antibody-producing cells and is representative of the performance retained in the biotinylated format of the Human IgA2 Antibody / Biotinylated Anti-IgA2 Detection Antibody. Heat-induced epitope retrieval was performed using pH6 citrate buffer or pH9 Tris-EDTA buffer prior to antibody incubation.

Human IgA2 Antibody Biotin ELISA Subclass Specificity Analysis. ELISA analysis of human immunoglobulins demonstrates that the parent clone RM125 antibody selectively recognizes Human IgA2 / IGHA2, with strong signal observed across tested concentrations, indicating specific binding to the alpha 2 heavy chain. No detectable cross-reactivity is observed with IgA1 or other immunoglobulin classes including IgG1, IgG2, IgG3, IgG4, IgM, IgD, or IgE. This binding profile reflects highly selective IgA2 recognition and is representative of the performance retained in the biotinylated format of the Human IgA2 Antibody for ELISA / Biotinylated Anti-IgA2 Detection Antibody.

Human IgA2 Antibody Biotin ELISA Titration Curve. ELISA titration using plates coated with serial dilutions of human IgA2 demonstrates strong, concentration-dependent binding of clone RM125 across a broad dynamic range. Signal intensity decreases proportionally with antibody dilution and antigen concentration, confirming consistent binding kinetics and high sensitivity. Detection was performed using an alkaline phosphatase-conjugated anti-rabbit IgG secondary antibody. This titration profile reflects the intrinsic binding performance of the parent clone RM125 antibody and is representative of the characteristics retained in the biotinylated format of the Human IgA2 Antibody for ELISA / Biotinylated Anti-IgA2 Detection Antibody.