Human IgA2 Antibody for ELISA Biotin Conjugate [clone RM125] (R20184BTN)

Sandwich ELISA of human IgA2 using recombinant Human IgA2 antibody as the capture (100ng/well), and biotinylated anti-human light chains (κ+λ) antibody clone RM129 as the detect, followed by an alkaline phosphatase conjugated streptavidin.

Sandwich ELISA of human plasma using recombinant Human IgA2 antibody as the capture (100ng/well), and biotinylated anti-human light chains (κ+λ) antibody clone RM129 as the detect, followed by an alkaline phosphatase conjugated streptavidin.

Recombinant Human IgA2 Antibody RM125 Biotin Conjugate in human tonsil by immunohistochemistry. Formalin-fixed paraffin-embedded human tonsil tissue stained with Recombinant Human IgA2 Antibody RM125 Biotin Conjugate shows HRP-DAB brown cytoplasmic staining in scattered plasma cells primarily located in interfollicular regions surrounding lymphoid follicles, consistent with expression of Immunoglobulin alpha 2 heavy chain (IGHA2), the defining heavy chain of the IgA2 antibody subclass. Most surrounding lymphocytes within the follicular structures remain negative. Heat induced epitope retrieval using either pH6 citrate buffer or pH9 Tris-EDTA buffer is recommended for FFPE tissue sections.

Human IgA2 Antibody for ELISA Biotin Conjugate clone RM125 specificity by ELISA. ELISA analysis of purified human immunoglobulins coated at 50 ng per well demonstrates the binding specificity of Human IgA2 Antibody for ELISA Biotin Conjugate clone RM125. Strong signal is observed with human IgA2 across tested antibody concentrations (200 ng/mL, 50 ng/mL, and 10 ng/mL), indicating selective recognition of the Immunoglobulin alpha 2 heavy chain (IGHA2). No detectable binding is observed with other immunoglobulin classes or subclasses including IgA1, IgG subclasses (IgG1, IgG2, IgG3, IgG4), IgM, IgD, or IgE, demonstrating high specificity of clone RM125 for the IgA2 subclass.

ELISA Titration: the plate was coated with different amounts of human IgA2. A serial dilution of recombinant Human IgA2 antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.