- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Recombinant Human IgA1 Antibody [clone RM124] (R20183)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
R20183-100UG | 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide | 100 ug | 429 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG |
| Clone Name | RM124 |
| Purity | Protein A purified from animal origin-free supernatant |
| UniProt | P01876 |
| Gene ID | 3493 |
| Applications | ELISA : 50ng/well-200ng/well (Capture); 0.05-0.2ug/ml (Detection) Immunocytochemistry : 0.5-2ug/ml Immunohistochemistry : 0.1-1ug/ml |
| Limitations | This recombinant Human IgA1 antibody is available for research use only. |
|
Review this product on BioCompare and get a $20 Amazon gift card
|
Related Products
|
The Recombinant Human IgA1 antibody is produced as a recombinant reagent that represents the IgA1 subclass of human immunoglobulin A. IgA1 is the predominant subclass in human serum and mucosal secretions, accounting for the majority of IgA found at epithelial surfaces. It plays an essential role in immune defense by neutralizing pathogens and toxins at mucosal barriers, thereby preventing colonization and infection. The Recombinant Human IgA1 antibody preserves the structural features of this subclass while being engineered without antigen specificity, providing an important isotype control and reference reagent in immunoassays.
Structurally, IgA1 consists of two heavy and two light chains, forming the classic Y shaped antibody structure. It contains a hinge region that is longer than that of IgA2, giving it greater flexibility but also making it more susceptible to cleavage by bacterial proteases. The Fc portion of IgA1 binds to FcαRI receptors, enabling effector functions such as phagocytosis and cytokine release. The Recombinant Human IgA1 antibody replicates these features but lacks antigen recognition, allowing it to serve as a negative control for background staining and nonspecific interactions in experimental systems.
The Recombinant Human IgA1 antibody is widely used in ELISA to verify assay specificity and to distinguish between true antigen antibody binding and nonspecific adherence. In flow cytometry, it provides baseline fluorescence controls and identifies nonspecific binding to Fcα receptors. In immunohistochemistry, the Recombinant Human IgA1 antibody highlights background reactivity in mucosal and lymphoid tissues. Recombinant production ensures lot to lot consistency, avoiding variability associated with hybridoma derived or serum derived reagents.
This reagent is also valuable in assay development and optimization, serving as a substitute during method refinement or secondary antibody validation. In clinical and translational research, analysis of IgA1 expression is important for understanding host defense at mucosal sites. IgA1 deficiencies or imbalances with IgA2 can contribute to recurrent infections, autoimmune disorders, and inflammatory diseases. Synonym terms such as recombinant human immunoglobulin A1 antibody and recombinant IgA1 isotype control antibody improve accessibility for users searching under alternate nomenclature.
By providing validated and reproducible detection, the Recombinant Human IgA1 antibody supports accurate interpretation of immunoassay results. NSJ Bioreagents ensures strict quality control for this reagent, giving investigators confidence in its consistent performance across multiple applications. With the Recombinant Human IgA1 antibody, researchers can reliably differentiate antigen specific responses from nonspecific background, strengthening studies of mucosal immunity and systemic immunology.
This recombinant Human IgA1 antibody reacts to human IgA1, and very slightly cross reacts with IgA2. No cross reactivity with human IgG, IgM, IgD, or IgE.
The stated application concentrations are suggested starting points. Titration of the recombinant Human IgA1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Human IgA was used as the immunogen for this recombinant Human IgA1 antibody.
Store the recombinant Human IgA1 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
Your bulk quote request has been submitted successfully!
Please contact us if you have any questions.
Powered by Bioz
Citations(3)