Human IgA1 Antibody for ELISA | Immunoglobulin alpha 1 heavy chain [clone RM124] (R20183)

Human IgA1 Antibody for ELISA clone RM124 sandwich ELISA detection of human plasma IgA1. Sandwich ELISA analysis of human plasma using Human IgA1 Antibody for ELISA clone RM124 as the capture antibody (100 ng per well). Detection was performed using biotinylated anti-human light chains (kappa+lambda) antibody clone RM129, followed by alkaline phosphatase conjugated streptavidin for signal development. The assay demonstrates concentration dependent detection of IgA1 in serial dilutions of human plasma, confirming effective capture by clone RM124 and detection through the light chain specific antibody.

Sandwich ELISA of human IgA1 using the recombinant Human IgA1 antibody as the capture (100ng/well), and biotinylated anti-human light chains (κ+λ) antibody clone RM129 as the detect, followed by an alkaline phosphatase conjugated streptavidin.

IHC testing of FFPE human tonsil tissue with recombinant Human IgA1 antibody. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Human IgA1 Antibody for ELISA clone RM124 specificity by ELISA. ELISA analysis of purified human immunoglobulins coated at 50 ng per well demonstrates the binding specificity of Human IgA1 Antibody for ELISA clone RM124. Strong signal is observed with human IgA1 across tested concentrations (200 ng/mL, 50 ng/mL, and 10 ng/mL), indicating selective recognition of the Immunoglobulin alpha 1 heavy chain (IGHA1). Very slight cross reactivity with IgA2 is observed, while no detectable binding occurs with other immunoglobulin classes including IgG, IgM, IgD, or IgE. These results indicate that clone RM124 preferentially recognizes IgA1 with minimal reactivity toward IgA2 and no cross reactivity with other human immunoglobulins.

Human IgA1 Antibody for ELISA clone RM124 titration by ELISA. ELISA titration analysis of Human IgA1 Antibody for ELISA clone RM124 using plates coated with varying amounts of purified human IgA1 (50 uL per well coating). A serial dilution of the recombinant rabbit monoclonal antibody RM124 was applied as the primary antibody, followed by an alkaline phosphatase conjugated anti-rabbit IgG secondary antibody for detection. The assay demonstrates concentration-dependent binding of the antibody to human IgA1, with higher absorbance values observed at greater coating concentrations and higher primary antibody concentrations. These results confirm robust and specific recognition of the Immunoglobulin alpha 1 heavy chain (IGHA1) by clone RM124 in ELISA-based detection assays.