Human IgA1 Antibody for ELISA / Anti-IgA1 Detection Antibody [clone RM124] (R20183)

Human IgA1 Antibody for ELISA Sandwich ELISA Human Plasma. Sandwich ELISA analysis demonstrates that clone RM124 functions effectively as a capture antibody for Human IgA1 / IGHA1, with signal intensity decreasing proportionally with plasma dilution, indicating strong and concentration-dependent detection. Captured IgA1 was detected using a biotinylated anti-human light chains (kappa + lambda) antibody (clone RM129), followed by alkaline phosphatase-conjugated streptavidin for signal development. This assay supports reliable detection of IgA1 in plasma and highlights the utility of this Human IgA1 Antibody for ELISA / Anti-IgA1 Detection Antibody for subclass-specific ELISA-based immunoassays.

Human IgA1 Antibody for ELISA Sandwich ELISA Human IgA1. Sandwich ELISA analysis using purified human IgA1 demonstrates that clone RM124 functions effectively as a capture antibody for Human IgA1 / IGHA1, with signal intensity decreasing proportionally with antigen concentration, indicating strong and concentration-dependent detection. Captured IgA1 was detected using a biotinylated anti-human light chains (kappa + lambda) antibody (clone RM129), followed by alkaline phosphatase-conjugated streptavidin for signal development. This assay configuration supports accurate quantification of IgA1 and highlights the utility of this Human IgA1 Antibody for ELISA / Anti-IgA1 Detection Antibody in subclass-specific ELISA-based immunoassays.

Human IgA1 Antibody Immunohistochemistry Human Tonsil Tissue. Immunohistochemistry analysis of FFPE human tonsil tissue using Human IgA1 Antibody / Anti-IgA1 Detection Antibody (clone RM124) demonstrates cytoplasmic staining in plasma cells localized within germinal center and interfollicular regions, while surrounding lymphocytes show minimal staining. The staining pattern supports detection of IgA1 / IGHA1 in antibody-secreting cells and is consistent with systemic IgA1-producing populations in lymphoid tissue. Heat-induced epitope retrieval was performed using pH6 citrate buffer or pH9 Tris-EDTA buffer prior to antibody incubation.

Human IgA1 Antibody for ELISA Subclass Specificity Analysis. ELISA analysis of human immunoglobulins demonstrates that clone RM124 selectively recognizes Human IgA1 / IGHA1, with strong signal observed across tested concentrations, indicating specific binding to the alpha 1 heavy chain. Minimal cross-reactivity with IgA2 is observed, while no detectable binding occurs with other immunoglobulin classes including IgG, IgM, IgD, or IgE. This binding profile supports preferential IgA1 detection and highlights the utility of this Human IgA1 Antibody for ELISA / Anti-IgA1 Detection Antibody for subclass-specific ELISA-based immunoassays.

Human IgA1 Antibody for ELISA Titration Curve. ELISA titration analysis using plates coated with serial dilutions of human IgA1 demonstrates strong, concentration-dependent binding of clone RM124 across a broad dynamic range. Signal intensity decreases proportionally with antibody dilution and antigen concentration, confirming high sensitivity and consistent binding performance. Detection was performed using an alkaline phosphatase-conjugated anti-rabbit IgG secondary antibody, supporting reliable quantification of Human IgA1 / IGHA1 in ELISA-based immunoassays.