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Home >> Antibodies >> Recombinant Human IgA1 Antibody

Recombinant Human IgA1 Antibody [clone RM124] (R20183)

  Catalog No Formulation Size Price (USD)  
Image R20183-100UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ug 419
Microvalidated Recrabbitmono
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Sandwich ELISA of human plasma using the recombinant human plasma antibody as the capture (100ng/well), and biotinylated anti-human light chains (κ+λ) antibody clone RM129 as the detect, followed by an alkaline phosphatase conjugated streptavidin.
Sandwich ELISA of human IgA1 using the recombinant Human IgA1 antibody as the capture (100ng/well), and biotinylated anti-human light chains (κ+λ) antibody clone RM129 as the detect, followed by an alkaline phosphatase conjugated streptavidin.
IHC testing of FFPE human tonsil tissue with recombinant Human IgA1 antibody. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.
ELISA of human immunoglobulins shows the recombinant Human IgA1 antibody reacts only to IgA. Very slightly cross reacts with IgA2. No cross reactivity with IgG, IgM, IgD, or IgE.
ELISA Titration: the plate was coated with different amounts of human IgA1. A serial dilution of recombinant Human IgA1 antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM124
Purity Protein A purified from animal origin-free supernatant
UniProt P01876
Gene ID 3493
Applications ELISA : 50ng/well-200ng/well (Capture); 0.05-0.2ug/ml (Detection)
Immunocytochemistry : 0.5-2ug/ml
Immunohistochemistry : 0.1-1ug/ml
Limitations This recombinant Human IgA1 antibody is available for research use only.
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Description

This recombinant Human IgA1 antibody reacts to human IgA1, and very slightly cross reacts with IgA2. No cross reactivity with human IgG, IgM, IgD, or IgE.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Human IgA1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Human IgA was used as the immunogen for this recombinant Human IgA1 antibody.

Storage

Store the recombinant Human IgA1 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

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