Human IgA1 Antibody for ELISA / Biotinylated Anti-IgA1 Detection Antibody [clone RM124] (R20183BTN)

Human IgA1 Antibody for ELISA Sandwich ELISA Human IgA1. Sandwich ELISA analysis using purified human IgA1 demonstrates that clone RM124 functions effectively as a capture antibody for Human IgA1 / IGHA1, with signal intensity decreasing proportionally with antigen concentration, indicating strong and concentration-dependent detection. Captured IgA1 was detected using a biotinylated anti-human light chains (kappa + lambda) antibody (clone RM129), followed by alkaline phosphatase-conjugated streptavidin for signal development. This assay configuration supports accurate quantification of IgA1 and highlights the utility of this Human IgA1 Antibody for ELISA / Anti-IgA1 Detection Antibody in subclass-specific ELISA-based immunoassays.

Human IgA1 Antibody Biotin Sandwich ELISA Human Plasma. Sandwich ELISA analysis demonstrates that the parent clone RM124 antibody functions effectively as a capture antibody for Human IgA1 / IGHA1, with signal intensity decreasing proportionally with plasma dilution, indicating strong and concentration-dependent detection. Captured IgA1 was detected using a biotinylated anti-human light chains (kappa + lambda) antibody (clone RM129), followed by alkaline phosphatase-conjugated streptavidin for signal development. This binding profile reflects the intrinsic performance of clone RM124 and is representative of the characteristics retained in the biotinylated format of the Human IgA1 Antibody for ELISA / Biotinylated Anti-IgA1 Detection Antibody.

Human IgA1 Antibody Biotin Immunohistochemistry Human Tonsil Tissue. Immunohistochemistry analysis of FFPE human tonsil tissue demonstrates that the parent clone RM124 antibody produces cytoplasmic staining in plasma cells distributed within germinal center and interfollicular regions, with minimal background in surrounding lymphoid cells. This staining pattern supports detection of IgA1 / IGHA1 in antibody-producing cells and is representative of the performance retained in the biotinylated format of the Human IgA1 Antibody / Biotinylated Anti-IgA1 Detection Antibody. Heat-induced epitope retrieval was performed using pH6 citrate buffer or pH9 Tris-EDTA buffer prior to antibody incubation.

Human IgA1 Antibody Biotin ELISA Subclass Specificity Analysis. ELISA analysis of human immunoglobulins demonstrates that the parent clone RM124 antibody selectively recognizes Human IgA1 / IGHA1, with strong signal observed across tested concentrations, indicating specific binding to the alpha 1 heavy chain. Minimal cross-reactivity with IgA2 is observed, while no detectable binding occurs with other immunoglobulin classes including IgG, IgM, IgD, or IgE. This binding profile reflects preferential IgA1 recognition and is representative of the performance retained in the biotinylated format of the Human IgA1 Antibody for ELISA / Biotinylated Anti-IgA1 Detection Antibody.

Human IgA1 Antibody Biotin ELISA Titration Curve. ELISA titration using plates coated with serial dilutions of human IgA1 demonstrates strong, concentration-dependent binding of clone RM124 across a broad dynamic range. Signal intensity decreases proportionally with antibody dilution and antigen concentration, confirming consistent binding kinetics and high sensitivity. Detection was performed using an alkaline phosphatase-conjugated anti-rabbit IgG secondary antibody. This titration profile reflects the intrinsic binding performance of the parent clone RM124 antibody and is representative of the characteristics retained in the biotinylated format of the Human IgA1 Antibody for ELISA / Biotinylated Anti-IgA1 Detection Antibody.