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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Human IgA1 Antibody Biotin Conjugate for ELISA [clone RM124] (R20183BTN)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
R20183BTN-50UG | 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide | 50 ug | 439 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Biotin Conjugate |
| Host | Rabbit |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG |
| Clone Name | RM124 |
| Purity | Protein A purified from animal origin-free supernatant |
| UniProt | P01876 |
| Gene ID | 3493 |
| Applications | ELISA : 50ng/well-200ng/well (Capture); 0.05-0.2ug/ml (Detection) Immunocytochemistry : 0.5-2ug/ml Immunohistochemistry : 0.1-1ug/ml |
| Limitations | This Human IgA1 antibody is available for research use only. |
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Immunoglobulin alpha 1 heavy chain (IGHA1) is the defining heavy chain component of the IgA1 subclass of immunoglobulin A antibodies. IgA antibodies are produced by plasma cells and function as an important component of humoral immunity, helping protect mucosal surfaces and systemic compartments from pathogens. The Human IgA1 Antibody Biotin Conjugate for ELISA clone RM124 recognizes the alpha 1 heavy chain of human IgA and enables subclass-specific detection of IgA1 antibodies in immunoassay systems. Biotin conjugation allows sensitive signal amplification through avidin or streptavidin based detection strategies commonly used in ELISA and related assay platforms.
IgA occurs in two subclasses in humans, IgA1 and IgA2, which differ in hinge region structure and distribution in biological fluids. IgA1 contains a longer hinge region that confers increased flexibility for antigen binding but also makes the molecule more susceptible to cleavage by certain bacterial proteases. As a result, IgA1 is the predominant IgA subclass found in serum and many extracellular fluids, whereas IgA2 is more commonly enriched in mucosal secretions where resistance to microbial proteases is advantageous. The IGHA1 gene encodes the alpha 1 heavy chain responsible for the structural properties and antigen recognition characteristics of IgA1 antibodies.
IgA1 antibodies are produced by plasma cells within lymphoid tissues including spleen, lymph nodes, bone marrow, and mucosa-associated lymphoid tissue. These antibodies participate in immune defense by binding pathogens and toxins, facilitating immune clearance and limiting microbial colonization. In mucosal environments polymeric IgA molecules can be transported across epithelial cells through interaction with the polymeric immunoglobulin receptor, generating secretory IgA complexes that function in immune exclusion mechanisms at epithelial surfaces.
Because IgA subclass distribution can vary depending on immune responses and disease conditions, detection of Immunoglobulin alpha 1 heavy chain expression provides valuable information for studying antibody-mediated immunity. An IgA1 antibody enables subclass-specific measurement of IgA responses in biological samples such as serum or plasma. Biotin-conjugated antibodies such as clone RM124 are particularly useful in ELISA assays where biotin-streptavidin amplification strategies improve assay sensitivity and enable precise quantification of IgA1 antibodies in immunological research.
The stated application concentrations are suggested starting points. Titration of the Human IgA1 Antibody Biotin Conjugate for ELISA may be required due to differences in protocols and secondary/substrate sensitivity.
Human IgA was used as the immunogen for this recombinant Human IgA1 antibody.
Store the recombinant Human IgA1 antibody at -20oC.
IgA1 antibody, Immunoglobulin A1 antibody, IGHA1 antibody, IgA subclass 1 antibody, Ig alpha heavy chain 1 antibody
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