Human IgA Antibody for ELISA / Immunoglobulin alpha heavy chain [clone RM128] (R20182)

Human IgA Antibody for ELISA clone RM128 sandwich ELISA detection of human plasma IgA. Sandwich ELISA analysis of human plasma using Human IgA Antibody for ELISA clone RM128 as the capture antibody (100 ng per well). Detection was performed using biotinylated anti-human light chains (kappa+lambda) antibody clone RM129, followed by alkaline phosphatase conjugated streptavidin for signal development. The assay demonstrates concentration dependent detection of human IgA in serial dilutions of plasma, confirming efficient capture of IgA by clone RM128 and detection through the light chain specific antibody.

Human IgA Antibody for ELISA clone RM128 sandwich ELISA detection of human IgA. Sandwich ELISA analysis of purified human IgA using Human IgA Antibody for ELISA clone RM128 as the capture antibody (100 ng per well). Detection was performed using biotinylated anti-human light chains (kappa+lambda) antibody clone RM129, followed by alkaline phosphatase conjugated streptavidin for signal development. The assay demonstrates concentration dependent detection of human IgA across a dilution series, confirming effective capture of Immunoglobulin alpha heavy chain (IGHA) containing antibodies by clone RM128 and detection through the light chain specific antibody.

Human IgA Antibody for ELISA clone RM128 specificity by ELISA. ELISA analysis of purified human immunoglobulins coated at 50 ng per well demonstrates the binding specificity of Human IgA Antibody for ELISA clone RM128. Strong signal is observed with both human IgA1 and IgA2 across tested antibody concentrations (200 ng/mL, 50 ng/mL, and 10 ng/mL), indicating recognition of the Immunoglobulin alpha heavy chain (IGHA) shared by both IgA subclasses. No detectable binding is observed with other immunoglobulin classes including IgG subclasses (IgG1, IgG2, IgG3, IgG4), IgM, IgD, or IgE, confirming selective detection of human IgA without cross reactivity to other immunoglobulins.

ELISA Titration: the plate was coated with different amounts of human IgA. A serial dilution of the recombinant Human IgA antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.

IHC staining of FFEP human lymphoid tissue with recombinant Human IgA antibody, HRP-secondary and DAB substrate. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.