- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Recombinant Human IgA Antibody [clone RM128] (R20182)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
R20182-100UG | 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide | 100 ug | 429 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG |
| Clone Name | RM128 |
| Purity | Protein A purified from animal origin-free supernatant |
| UniProt | P01876 , P01877 |
| Gene ID | 3493 , 3494 |
| Applications | ELISA : 50ng/well-200ng/well (Capture); 0.05-0.2ug/ml (Detection) |
| Limitations | This recombinant Human IgA antibody is available for research use only. |
|
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The Recombinant Human IgA antibody is produced as a recombinant reagent that reflects the architecture and effector functions of human immunoglobulin A. IgA is the most abundant antibody isotype at mucosal surfaces and plays a critical role in protecting the respiratory, gastrointestinal, and genitourinary tracts. By neutralizing pathogens and toxins before they can penetrate epithelial barriers, IgA serves as the first line of adaptive immune defense. The Recombinant Human IgA antibody mimics the structure of this isotype while lacking antigen specificity, providing a reliable negative control and reference reagent for immunoassays.
Structurally, IgA exists in two main forms. In circulation, it is typically monomeric, whereas at mucosal sites, IgA is secreted as a dimer linked by a joining (J) chain and associated with a secretory component that facilitates transcytosis across epithelial cells. The Fc portion of IgA binds to the FcαRI receptor, enabling effector functions such as phagocytosis and release of inflammatory mediators. The Recombinant Human IgA antibody preserves these constant region properties while being engineered without variable domain specificity, ensuring it identifies nonspecific binding in experimental settings.
Applications of the Recombinant Human IgA antibody include ELISA, where it establishes assay specificity and confirms that signals originate from antigen antibody recognition rather than background adherence. In flow cytometry, it defines baseline fluorescence and controls for nonspecific Fcα receptor binding on immune cells. In immunohistochemistry, the antibody reveals background staining in tissues rich in mucosal IgA activity. Recombinant production ensures lot to lot consistency, overcoming variability that can affect hybridoma or serum derived reagents.
This antibody is also valuable for assay optimization, serving as a substitute reagent when refining blocking strategies, secondary antibody validation, or detection conditions. In clinical research, the ratio of IgA to other immunoglobulin isotypes is important for understanding immune competence, with deficiencies linked to recurrent infections and autoimmune disease. Having a standardized recombinant control such as the Recombinant Human IgA antibody ensures reproducibility across laboratories and enhances data comparability. Synonym terms such as recombinant human immunoglobulin A antibody and recombinant IgA isotype control antibody increase product visibility for diverse users.
By delivering validated and reproducible performance, the Recombinant Human IgA antibody enhances reliability in both experimental and translational applications. NSJ Bioreagents validates this reagent under strict quality standards, giving researchers confidence in its performance across flow cytometry, ELISA, and histology. With the Recombinant Human IgA antibody, scientists can reliably separate antigen specific signals from nonspecific background, strengthening studies of mucosal immunity and systemic immunology.
This recombinant Human IgA antibody reacts to human IgA, including both IgA1 and IgA2. No cross reactivity with human IgG, IgM, IgD, or IgE.
The stated application concentrations are suggested starting points. Titration of the recombinant Human IgA antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Human IgA was used as the immunogen for this recombinant Human IgA antibody.
Store the recombinant Human IgA antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
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