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Home >> Antibodies >> Recombinant Human IgA Antibody (Biotin Conjugate)

Recombinant Human IgA Antibody (Biotin Conjugate) [clone RM128] (R20182BTN)

  Catalog No Formulation Size Price (USD)  
Image R20182BTN-50UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 50 ug 379
Sandwich ELISA of human IgA using the recombinant Human IgA antibody as the capture (100ng/well), and biotinylated anti-human light chains (κ+λ) antibody, clone RM129, as the detect, followed by an alkaline phosphatase conjugated streptavidin.
Sandwich ELISA of human plasma using the Recombinant Human IgA Antibody as the capture (100ng/well), and biotinylated anti-human light chains (κ+λ) antibody, clone RM129, as the detect, followed by an alkaline phosphatase conjugated streptavidin.
IHC testing of FFPE human lymphoid tissue with recombinant Human IgA antibody. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.
ELISA of human immunoglobulins shows the recombinant Human IgA antibody reacts to both Human IgA1 & IgA2. No cross reactivity with Human IgG, IgM, IgD, or IgE.
ELISA Titration: the plate was coated with different amounts of human IgA. A serial dilution of the recombinant Human IgA antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
Availability 1-3 business days
Species Reactivity Human
Format Biotin Conjugate
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM128
Purity Protein A purified from animal origin-free supernatant
UniProt P01876 , P01877
Gene ID 3493 , 3494
Applications ELISA : 50ng/well-200ng/well (Capture); 0.05-0.2ug/ml (Detection)
Immunocytochemistry : 0.5-2ug/ml
Immunohistochemistry : 0.1-1ug/ml
Limitations This recombinant Human IgA antibody is available for research use only.
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Description

This recombinant Human IgA antibody reacts to human IgA, including both IgA1 and IgA2. No cross reactivity with human IgG, IgM, IgD, or IgE.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Human IgA antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Human IgA was used as the immunogen for this recombinant Human IgA antibody.

Storage

Store the recombinant Human IgA antibody at -20oC.

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