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Home >> Antibodies >> Recombinant HSP60 Antibody

Recombinant HSP60 Antibody [clone HSPD1/6496R] (V9279)

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  Catalog No Formulation Size Price (USD)  
Image V9279-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V9279-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V9279SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recrabbitmono
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Western blot testing of human Hep2 cell lysate using recombinant HSP60 antibody (clone HSPD1/6496R). Predicted molecular weight: ~60 kDa.
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Western blot testing of human Hep2 cell lysate using recombinant HSP60 antibody (clone HSPD1/6496R). Predicted molecular weight: ~60 kDa.
IHC staining of FFPE human liver tissue with recombinant HSP60 antibody (clone HSPD1/6496R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name HSPD1/6496R
Purity Protein A/G affinity
UniProt P10809
Localization Cytoplasm
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This recombinant HSP60 antibody is available for research use only.
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Description

Recognizes a 60kDa protein, identified as the heat shock protein 60 (hsp60). Its epitope is localized between aa 383-447 of human hsp60. A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsp s), more appropriately called as stress response proteins (srp s). hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial hsp60, but also against its mammalian homolog. Clone HSPD1/6496R, unlike clone LK2, recognizes only the mammalian (not bacterial) hsp60 and is useful in distinguishing hsp60 from mammals and bacteria.

Application Notes

Optimal dilution of the recombinant HSP60 antibody should be determined by the researcher.

Immunogen

Recombinant human full-length HSP60 protein was used as the immunogen for the recombinant HSP60 antibody. Its epitope is localized between amino acids 383-447 of human hsp60.

Storage

Aliquot the recombinant HSP60 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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