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Home >> Antibodies >> Recombinant HPV16 L1 Antibody / Rabbit Monoclonal

Recombinant HPV16 L1 Antibody / Rabbit Monoclonal [clone HPV16/2058R] (V3660)

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  Catalog No Formulation Size Price (USD)  
Image V3660-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3660-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3660SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V3660IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
Microvalidated Recrabbitmono
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IHC staining of FFPE human cervix with recombinant HPV16 L1 antibody (clone HPV16/2058R). Required HIER: boil tissue sections in pH9 EDTA buffer for 45 min followed by cooling at RT for 20 min.
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IHC staining of FFPE human cervix with recombinant HPV16 L1 antibody (clone HPV16/2058R). Required HIER: boil tissue sections in pH9 EDTA buffer for 45 min followed by cooling at RT for 20 min.
IHC staining of FFPE human cervix with recombinant HPV16 L1 antibody (clone HPV16/2058R). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE analysis of purified, BSA-free recombinant HPV16 L1 antibody (clone HPV16/2058R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Type 16 of Human Papilloma Virus (HPV-16)
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name HPV16/2058R
Purity Protein A affinity chromatography
UniProt P03101
Localization Nuclear
Applications Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT
Limitations This recombinant HPV16 L1 antibody is available for research use only.
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Description

Reacts with a protein of 57kDa, identified as the L1 protein of human papilloma virus type 16 (HPV-16). It is the major capsid protein of HPV-16. Infection with specific types of HPV has been associated with an increased risk of developing cervical neoplasia. HPV types 6 and 11 have been associated with relatively benign diseases such as genital warts but types 16 and 18 are strongly associated with cervical, vaginal, and vulvar malignancies. The antibody reacts very strongly with formalin-fixed, paraffin-embedded tissues containing HPV-16 or -33; very weak reactions were occasionally observed with biopsy specimens or smears containing HPV-6 or HPV-11. It cross-reacts with HPV37.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant HPV16 L1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Human papilloma virus type 16, major capsid protein L1, was used as the immunogen for the recombinant HPV16 L1 antibody.

Storage

Store the recombinant HPV16 L1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Related Products

   1.   View all HPV16 L1 antibodies

   2.   View all recombinant antibodies

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