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Home >> Antibodies >> Recombinant Histone H3 Antibody

Recombinant Histone H3 Antibody [clone RM186] (R20228)

  Catalog No Formulation Size Price (USD)  
Image R20228-100UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ug 469
Image
R20228-25UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 25 ug 239
Microvalidated Recrabbitmono
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Western blot test of acid extracts of HeLa cells treated with sodium butyrate (HeLa+NaBu), treated and untreated with Nocodazole, and recombinant Histone H3.1 protein, using recombinant Histone H3 antibody at 1 ug/ml, showed a band of Histone H3 with unmodified Lys4.
ICC/IF staining of HeLa cells treated with sodium butyrate using recombinant Histone H3 antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).
This recombinant Histone H3 antibody specifically recognizes Histone H3 unmodified at Lys4 and does not recognize acetylated, monomethylated, dimethylated, or trimethylated Lys4. The mAb binding specificity allows for modifications of Arg2 or Thr3 in Histone H3.
Availability 1-3 business days
Species Reactivity All Species
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM186
Purity Protein A purified from animal origin-free supernatant
UniProt Q16695
Gene ID 8350
Applications Western blot : 1-2ug/ml
ELISA : 0.5-1ug/ml
Immunocytochemistry : 0.5-2ug/ml
Limitations This recombinant Histone H3 antibody is available for research use only.
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Description

This recombinant Histone H3 antibody recognizes Histone H3 that is unmodified at Lys4 but does not recognize acetylated or methylated Lys4. The recombinant Histone H3 antibody binding specificity allows for modifications of Arg2, Thr3, and/or other modifications in Histone H3.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Histone H3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

An unmodified peptide corresponding to the N-terminus of Histone H3 was used as the immunogen for this recombinant Histone H3 antibody.

Storage

Store the recombinant Histone H3 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

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