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Home >> Antibodies >> Recombinant Histone H2AX Antibody / H2AFX

Recombinant Histone H2AX Antibody / H2AFX [clone RM214] (R20250)

  Catalog No Formulation Size Price (USD)  
Image R20250-100UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ug 469
Image
R20250-25UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 25 ug 239
Microvalidated Recrabbitmono
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Western blot of A375, HEK293, HeLa and SK-MEL-2 whole cell lysates using recombinant Histone H2AX antibody at 0.5 ug/ml. Predicted molecular weight ~15 kDa.
ICC/IF staining of human HeLa cells using recombinant Histone H2AX antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).
Availability 1-3 business days
Species Reactivity All Species
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM214
Purity Protein A purified from animal origin-free supernatant
UniProt P16104
Gene ID 3014
Applications Western blot : 0.5-2ug/ml
Immunocytochemistry : 1-2ug/ml
ELISA : 0.2-1ug/ml
Limitations This recombinant Histone H2AX antibody is available for research use only.
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Description

This recombinant Histone H2AX antibody reacts to Histone H2AX protein, independent of post-translational modifications. No cross reactivity with other histone proteins.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Histone H2AX antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A peptide corresponding to the C-terminus of human Histone H2AX was used as the immunogen for this recombinant Histone H2AX antibody.

Storage

Store the recombinant Histone H2AX antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

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