- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Histone H1 Antibody / Chromatin Accessibility Linker Histone Antibody [clone OSHT-3R] (V3689)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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V3689-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 100 ug | 559 | |
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V3689-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 20 ug | 259 | |
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V3689SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 | |
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V3689IHC-7ML | Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* | 7 ml | 559 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Rabbit |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG, kappa |
| Clone Name | OSHT-3R |
| Purity | Protein A affinity chromatography |
| UniProt | P07305 |
| Localization | Nuclear |
| Applications | Flow Cytometry : 1-2ug/10^6 cells Immunofluorescence : 1-2ug/ml Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RTPrediluted IHC only format |
| Limitations | This recombinant Histone H1 antibody is available for research use only. |
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Histone H1 is a linker histone that plays a central role in regulating chromatin accessibility by controlling DNA packaging and higher-order chromatin organization. Histone H1 Antibody detects H1 protein associated with chromatin states that influence DNA accessibility, positioning histone H1 as a key regulator of how tightly DNA is packaged within the nucleus. This function distinguishes histone H1 from core histones by emphasizing its role in modulating genome accessibility rather than forming nucleosome structure. This antibody is part of our broader Histone H1 antibody collection, including linker histone variants, chromatin organization, chromatin accessibility, and nuclear architecture reagents for chromatin and epigenetics research.
Histone H1 antibody, also referred to as H1 antibody or linker histone antibody in the literature, is widely used to study the relationship between chromatin structure and DNA accessibility. By binding to linker DNA between nucleosomes, histone H1 stabilizes nucleosome arrays and promotes chromatin compaction, thereby limiting access of cellular machinery to DNA. This regulatory function makes histone H1 an important determinant of genome accessibility at a global level.
Mechanistically, histone H1 influences chromatin accessibility through its effects on nucleosome arrangement and chromatin folding. Increased association of histone H1 is typically correlated with reduced DNA accessibility, while decreased H1 levels are associated with more open chromatin configurations. This dynamic balance allows chromatin to shift between more compact and more accessible states depending on cellular context.
Histone H1-mediated regulation of chromatin accessibility is essential for controlling genome interactions and maintaining proper chromatin organization. By modulating DNA packaging, histone H1 influences the ability of cellular factors to interact with chromatin, thereby contributing to regulation of genome function at the structural level.
The distribution of histone H1 across chromatin is highly dynamic and responsive to cellular conditions, including changes in chromatin state and environmental signals. Redistribution of H1 can alter chromatin accessibility and impact genome organization, highlighting its role as a regulator of chromatin structure.
In contrast to chromatin condensation-focused roles of histone H1, its function in chromatin accessibility emphasizes the balance between DNA compaction and structural flexibility. This distinction is important for understanding how chromatin structure supports both genome stability and regulated genome interactions.
A recombinant rabbit monoclonal antibody targeting histone H1 enables specific and consistent detection of linker histone involved in chromatin accessibility regulation, supporting studies of chromatin structure, genome organization, and DNA accessibility dynamics.
The stated application concentrations are suggested starting points. Titration of the Histone H1 Antibody / Chromatin Accessibility Linker Histone Antibody may be required due to differences in protocols and secondary/substrate sensitivity.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Recombinant full-length human protein was used as the immunogen for the Histone H1 Antibody / Chromatin Accessibility Linker Histone Antibody.
Store the recombinant Histone H1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Histone H1 antibody, H1 antibody, chromatin accessibility histone H1 antibody, linker histone accessibility antibody
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