Recombinant Histone H1 Antibody Rabbit Monoclonal HH1/1784R

	Catalog No	Formulation	Size	Price (USD)
	V7278-100UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	100 ug	519
Formulation
	V7278-20UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	20 ug	229
Formulation
	V7278SAF-100UG	1 mg/ml in 1X PBS; BSA free, sodium azide free	100 ug	519
Formulation
	V7278IHC-7ML	Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; For IHC use only	7 ml	519
Formulation

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IHC testing of FFPE human pancreas and recombinant Histone H1 antibody (clone HH1/1784R). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.

Immunofluorescent staining of permeabilized human HeLa cells with recombinant Histone H1 antibody (green, clone HH1/1784R) and Phalloidin.

Flow cytometry testing of PFA-fixed human HeLa cells with recombinant Histone H1 antibody (clone HH1/1784R); Red=isotype control, Blue= recombinant Histone H1 antibody.

SDS-PAGE analysis of purified, BSA-free recombinant Histone H1 antibody (clone HH1/1784R) as confirmation of integrity and purity.

Western blot testing of human heart lysate with recombinant Histone H1 (clone HH1/1784R). Observed molecular weight ~22/30-33 kDa (unmodified/modified).

Availability	1-3 business days
Species Reactivity	Human, Mouse, Rat
Format	Purified
Clonality	Recombinant Rabbit Monoclonal
Isotype	Rabbit IgG, kappa
Clone Name	HH1/1784R
Purity	Protein A affinity chromatography
UniProt	P07305
Localization	Nuclear
Applications	Flow cytometry : 1-2ug/million cells Immunofluorescence : 1-2ug/ml Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT Western blot : 1-2ug/ml
Limitations	This recombinant Histone H1 antibody is available for research use only.
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Description

Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division. [UniProt]

Application Notes

Optimal dilution of the recombinant Histone H1 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.